摘要
我们分别采用Ca^(2+)和Mg^(2+)处理大肠杆菌JM109进行转化实验,使含有1.25——双羟胆钙化醇受体(1,25—Dihydroxy cholecalciferol receptor,DHCCR)基因的质粒转化到细菌中进行克隆获得成功。经氨苄青霉素(AMP)培养基上筛选及限制性酶切分析,证明转化子为阳性。
In the study, we successfully transformed recombinant DNA of 1. 25-Dihydroxy cholecalciferol receptor (DHCCR) into Escherichia Coli (E · Coli) JM109 treated by CaCl2 or MgCl2 to Construct pUC-DHCCR E · Coli clone. The positive transformant has been identified by screening test on agar plate with ampicillin and by analysis with restriction endonuclease. The result has a practical significance for further research on the properties of DHCCR and the preparation and storage of DHCCR recombinant DNA.
出处
《白求恩医科大学学报》
CSCD
1993年第3期235-236,共2页
Journal of Norman Bethune University of Medical Science
基金
国家自然科学基金
关键词
重组
DNA
细菌
转化
限制性内切酶
DNA, recombinant/BI
calcitrol/BI
transformation, bacterial
DNA restric-tio enzymes/BI
