Rb_1对培养心肌细胞抗氧化损伤作用

Antioxidative damage action of panaxadiol monomer Rb_1 on cultured myocardiocytes

采用电镜观察和电子自旅共振(ESR测定)的方法,我们观察了黄漂呤—黄嘌呤氧化酶(X—XOD)对培养新生Wistar大鼠的氧化损伤作用以及人参二醇组皂甙单体Rb_1抗氧化损伤作用。结果表明,X-XOD增加自由基数量,破坏肌丝和线粒体等作用。加入培养基中Rb_130μg/ml能明显对抗X-XOD引起的各种变化。

Using the methods of observation under electron microscopy and electron spin reso- nance spectroscopy (ESR). we observed the damage action of X -XOD and antioxidative damage action of panaxadiol saponin monomer Rb1 on the cultured myocardiocytes in neonatal Wistar rat. The results indicated that X - XOD destroyed the myofilaments and mitochondria and increased the amount of free radicals. Rb1 30μg/ml added to the medium could apparently antagonize all of the variations induced by X-XOD.