3-硝基丙酸预处理对多巴胺能神经元保护作用的研究

Investigation of protective effect on dopaminergic neuron of 3-NPA preconditioning

目的:研究3-硝基丙酸(3-NPA)预处理对MPP+(1-甲基4苯基-吡啶离子)损害的多巴胺能神经元是否具有保护作用。方法:将MPP+加入到培养的多巴胺能神经元SH-SY5Y细胞中制作帕金森病的细胞模型,在加入MPP+(0.25mmol/L)前,分别单次或多次加入3-NPA(0.2mmol/L)形成预处理,应用四氮唑盐(MTT)检测细胞生存率,[3H]DA摄取率测定多巴胺能细胞突触前功能,观察3-NPA预处理对它们的影响。结果:3-NPA预处理后,预处理组细胞生存率分别为71.8％(单次),85.2％(多次),较MPP+组(54.3％)明显提高;[3H]DA摄取率分别为65.8％(单次)80.3％(多次),较MPP+组(50.1％)明显提高,且多次预处理较单次预处理效果更好。单加3-NPA对细胞无影响。结论:3-NPA预处理对多巴胺能神经元有明显的保护作用,多次预处理的保护作用更加显著。

Objective: To investigate whether 3-NPA preconditioning could have protective effect on dopaminergic neuron induced by MPP+ . Methods; MPP+ (0. 25mm/L) was added into the cultured dopaminergic neuron SH-SY5Y cell to produce the Parkinson model. Before MPP+ added, 3-NPA (0. 2mm/L) was added( single time or repetive) for preconditioning. MTT was used to assay the survival of the cell and [ H ] DA uptake was detected to display the function of anterior synapse, so as to observe the influence of 3-NPA preconditioning on them. Results: The rate of survival cell without treated by 3-NPA was 71.8% (single time) and 85. 2% (repetive) respectively. The rate of survival cell without treated by 3-NPA was 54. 3% (MPP+ ). There were significant difference among them. The rate of [3H] DA uptake were 65. 8% (single time) , 80. 3% (repetive) and 50. 1 (MPP+ ) respectively. There were also significant difference among them. The effect of repetive preconditioning was better than single time. Conclusion; 3-NPA preconditioning had the protective effect on the dopaminergic neuron induced by MPP+ The content of protective effect of repetive preconditioning was obviously better than single preconditionging.