摘要
目的 :探讨早孕绒毛组织中IGF IImRNA表达水平的变化与胎盘、胚胎早期发育的关系。方法 :采用逆转录聚合酶链反应 (RT PCR)检测 2 0例正常早孕妇女 (正常对照组 )、15例自然流产妇女 (自然流产组 )及 2 0例药物流产妇女 (药物流产组 )绒毛组织中IGF IImRNA的表达 ,同时用PAS染色法分别观测其绒毛组织中糖原储存水平的变化 ,并进行半定量分析和相关性分析。结果 :(1)IGF IImRNA在自然流产组、药物流产组绒毛组织中的表达水平分别为 1.19± 0 .6 7、1.4 2± 0 .6 4,明显低于正常早孕组的 2 .2 2± 0 .95 ,差异均具有显著性 (P <0 .0 5 ) ,药物流产组与自然流产组差异无显著性 (P >0 .0 5 ) ;(2 ) 3组绒毛组织中糖原灰度值分别为 10 6 .0 4± 12 .6 0、82 .85± 2 2 .79、85 .94± 16 .15 ,自然流产组、药物流产组与正常对照组相比差异有显著性 (P <0 .0 5 ) ;(3)绒毛组织中IGF IImRNA的表达强度与其糖原储存水平呈正相关 (P <0 .0 5 )。结论 :推测早孕绒毛组织IGF IImR NA的适量表达与胎盘、胚胎发育有关 ,IGF对胎盘糖原合成的调节可能是其重要作用机制之一。
Objective:To investigate the effect of the expression of insulin-like growth factor-II(IGF-II) in human villus on the embryo's development in early pregnancy.Methods:Villus samples were obtained from 15 spontaneous abortion,20 mifepristone induced abortion and 20 normal early pregnancy.Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was utilized to measure the level of mRNA expression of IGF-II in three groups.Glycogen contenant in all samples were investigated by histochemistry and image analysis technology.Results:The levels of IGF-II mRNA in villus from two abortion groups were 1.19±0.67,1.42±0.64.They were both significantly decreased as compared with those from normal group(2.22±0.95)(P<0.05);(2)Glycogen contenant in three groups were 106.04±12.60,82.85±22.79,85.94±16.15 respectively.Compared with the normal control group,the two abortion groups' glycogen contenants were decresed too(P<0.05);(3)There was obvious correlation between IGF-II and glycogen contenant(P<0.05).Conclusion:The expression of IGF-II gene is related with the placenta and the embryo development in early pregnancy,and the regulation of IGF-II on glycogen contenant is possibly one of the major mechanisms.
出处
《现代妇产科进展》
CSCD
2004年第3期207-209,共3页
Progress in Obstetrics and Gynecology
关键词
胰岛素样生长因子-Ⅱ
妊娠初期
胚胎发育
Insulin-like growth factor II
Pregnancy trimester,first
Embryo development