长角血蜱卵黄蛋白的纯化及其性质

Purification and properties of vitellin from the bush tick, Haemaphysalis longicornis Neumann (Arachnida: Ixodidae)

用凝胶过滤与离子交换层析、蛋白质电泳和糖脂蛋白染色等方法提取纯化长角血蜱Haemaphysalislongicornis卵黄蛋白 ,并对其性质进行了研究。PAGE和SDS PAGE分析表明 ,长角血蜱的卵黄蛋白只有一种 ,由 8个亚基组成 ,亚基的相对分子质量分别为 112kD ,10 3kD ,80kD ,78kD ,71kD ,6 8kD ,6 2kD和 5 2kD ,卵黄蛋白经苏丹黑B和希夫试剂染色呈阳性 ,表明是一种含血红素的糖脂蛋白。

Vitellin was purified from the eggs of the bush tick, Haemaphysalis longicornis Neumann by gel filtration and ion exchange chromatography, and its property was also analyzed. PAGE and SDS-PAGE results showed that only one kind of vitellin existed in H. longicornis. The vitellin composed of eight subunits and their molecular weights were 112, 103, 80, 78, 71, 68, 62 and 52 kD respectively. The vitellin could be stained by Sudan black B and periodic acid-Schiff (PAS), which meant that it was an hemoglycolipoprotein.