摘要
测定了杀虫植物砂地柏SabinavulgarisAnt.的精油中主杀虫成分———松油烯 4 醇 (terpinen 4 ol)对粘虫MythimnaseparataWalker幼虫的生物活性。结果表明 ,松油烯 4 醇对粘虫主要表现为熏蒸作用 ,对粘虫 3龄幼虫 2 4h的熏蒸LC50 为 5 3473μL L ;还具一定触杀作用 ,对粘虫 4龄幼虫 2 4h的LD50 为 14 7 8μg 虫。试虫的中毒症状可明显地分为兴奋、痉挛、麻痹和死亡 4个阶段 ,而麻痹的部分试虫有复苏现象。可明显抑制Na+ ,K+ ATP酶的活性 ,在兴奋期、痉挛期、麻痹期和复苏期 ,抑制率介于2 1 2 8%~ 34 92 %之间。离体条件下对Na+ ,K+ ATP酶的I50 为 133 75 μg·mL- 1 ;对AChE活性有一定的影响 ;对酯酶 ,在兴奋期 ,酶活力为对照的 77 0 % ,在麻痹期则为对照的 1 33倍 ,而复苏期试虫的酯酶活力与对照相当。
Terpinen-4-ol is the main insecticidal composition in essential oil of Sabina vulgaris Ant.,an insecticidal plant. The toxicity, poisoning symptoms and biochemical effects of terpinen-4-ol on larvae of the oriental armyworm, Mythimna separata (Walker) were assayed. The results showed that terpinen-4-ol was very toxic to 3-instar larvae of the armyworm with fumigating application (LC_(50) =5.3473 μL·L^(1))and the LD_(50) of contact activity to 4-instars larvae of the armyworm was 147.8 μg/larvae. The poisoning symptom caused by the compound could be divided into 4 stages, i.e., excitation, convulsion, paralysis and death; but some paralyzed insects could recover. In vivo, the activity of Na^+,K^+-ATPase in brain of 5th instar larvae tested was inhibited; the inhibition ratio was 21.28% in exciting stage, 25.62% in convulsing stage, 32.10% in paralysis stage, and 34.92% in recovering stage, respectively. In vitro, the I_(50) of Na^+,K^+-ATPase was 133.75 μg·mL^(-1)(Y=2.2236+1.3058X r=0.988, χ~2=1.26). It had certain influence on the activity of AChE. In the excitation stage, the activity of ester enzyme of the treated larvae was 77.0% of that of the control; in the paralysis stage, it was 1.33 times; but it was as high as that of the control in the recovery stage.
出处
《昆虫学报》
CAS
CSCD
北大核心
2004年第3期329-333,共5页
Acta Entomologica Sinica
基金
国家"十五"科技攻关项目 (2 0 0 2BA5 16A0 4)
国家自然科学基金资助项目 (3 9970 5 0 6)
国家"863"项目 (2 0 0 1AA2 460 16)