云南桔小实蝇五个地理种群的遗传分化研究

Genetic differentiation in five geographic populations of the oriental fruit fly,Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) in Yunnan Province

对云南省瑞丽、景洪、化念、元江、河口的桔小实蝇 5个地理种群共 2 7个个体的线粒体细胞色素氧化酶Ⅰ (COⅠ )基因进行了部分序列测定。序列的碱基变化中转换明显多于颠换、无碱基的插入和缺失。 5个地理种群中共有 2 7个多态位点和2 3种单倍型 ,其中 2种为共享单倍型。桔小实蝇 5个地理种群的Fst值在 0 0 36 4～ 0 136 4之间 (P >0 0 5 ) ,Nm值在 3 88～13 2 5之间。对其所有单倍型聚类分析发现 ,单倍型在系统树中的分布散乱、混杂 ,没有显示出明显的地理分布族群。分析认为 5个地理种群间已存有一定程度的遗传分化 ,但分化的程度还比较低。造成 5个种群这种遗传分化的因素主要与地理隔离有关 。

Partial mitochondrial cytochrome oxidaseⅠ (COⅠ) region was sequenced from 27 individuals of 5 geographic populations of Bactrocera dorsalis (Hendel) from Ruili, Jinghong, Yuanjiang, Huanian and Hekou of Yunnan Province, respectively. Transition was higher than transversion, without insertion and deletion. In total, 23 haplotypes were identified within the sequences, with 27 sites showing polymorphism and 2 haplotypes shared. The Fst values among 5 populations were 0.0364-0.1364 (P>0.05) and Nm 3.88-13.25. Phylogenetic trees of haplotypes were constructed by N-J method. Haplotypes did not accord with their geography. All results showed that genetic differences existed among 5 populations, but the degree was lower. The main reason of the genetic differentiation among 5 populations should be related to genetic isolation, development history and ecology adaptation of Bactrocera dorsalis.