川芎嗪对骨髓移植小鼠CD44表达的作用研究

The Effect of Ligustrazine on the Expression of CD44 in Bone Marrow of Bone Marrow Transplantation Mice

为探讨川芎嗪对骨髓移植小鼠早期造血重建过程中黏附分子CD4 4表达水平的影响 ,将BALB c小鼠随机分为 3组 :正常对照组 ,骨髓移植对照组 (简称对照组 )和骨髓移植 +川芎嗪治疗组 (简称川芎嗪组 ) ,对照组和川芎嗪组每天分别胃饲生理盐水和川芎嗪。于骨髓移植 (BMT)后第 7,14 ,2 1,2 8天处死小鼠 ,计数外周血细胞、骨髓有核细胞 ,分析骨髓中造血组织面积及成熟红细胞容量 ,用流式细胞术和免疫组织化学检测骨髓细胞上CD4 4的表达水平 ,并于第 10天取小鼠脾脏 ,计数脾集落形成单位。结果显示 ,骨髓移植后第 7,14 ,2 1,2 8天川芎嗪组外周血白细胞、血小板、骨髓有核细胞计数、骨髓中造血组织面积均显著高于移植对照组 ,外周血红细胞计数在第 7,14 ,2 1天也较对照组为高 ;同时 ,川芎嗪组CFU S计数明显高于同期移植对照组 ,骨髓中成熟红细胞容量则显著低于对照组 ,川芎嗪组CD4 4的表达在第 7,14 ,2 1,2 8天明显高于对照组。结论 :川芎嗪可提高骨髓移植小鼠骨髓细胞表面CD4 4的表达水平 ,促进造血干 祖细胞的归巢 ,加速移植后骨髓的造血重建。

The aim of this study was to investigate the effects of ligustrazine on the expression of CD44 in bone marrow tissue at the early phase of hematopoietic reconstruction after bone marrow transplantation (BMT) in mice. The mice were divided into 3 groups: normal mice without BMT treatment,BMT only and BMT added with ligustrazine groups. On the days 7,14,21,28 after BMT,peripheral blood cells and bone marrow nucleated cells (BMNC) were counted,while the expression of CD44 was assayed by flow cytometry. CFU-S was determined on the day 10 after BMT. The results showed that in ligustrazine group the WBC,Plt and BMNC counts and hematopoietic tissue area estimation in bone marrow on the days 7,14,21,28 after BMT were all higher than in BMT controlled group,CFU-S counts were higher than in BMT only group too. On the contrary,mature RBC volume was lower than in the BMT only group. The expression of CD44 in ligustrazine group on days 7,14,21,28 after BMT was significantly higher than in BMT only group. It is concluded that ligustrazine can enhance CD44 expression in bone marrow of syngenic BMT mice,which may be one of mechanisms of ligustrazine accelerating hematopoietic reconstitution in bone marrow.