3438例山东汉族脐血供者HLA-DRB1等位基因频率的分布特点

Distribution Features of HLA-DRB1 Allele Frequencies in Normal Cord Blood Donors of 3 438 Shandong Hans

为研究山东汉族人群DRB1基因的分布特征 ,探讨中国广大地区甚至世界其它人种利用山东脐血供者进行造血干细胞移植的可能性 ,应用PCR SSP方法对山东地区 3438例无血缘关系的汉族健康新生儿脐血进行了HLA DRB1低分辨等位基因的分布调查。结果显示 :在山东汉族人群中前 5位高频率等位基因依次为DRB1 15(0 .1817) , 0 7(0 .136 9) , 0 9(0 .12 2 1) , 0 4 (0 .10 84 )和 12 (0 .10 38) ,低频率的等位基因是DRB1 0 30 2 0 30 3(0 .0 0 0 3) , 10 (0 .0 15 1) , 16 (0 .0 2 6 2 )和 0 1(0 .0 32 2 )。通过山东汉族人群与相关文献中其它人种和不同地域的汉族人群的比较分析显示 :没有任何 1个DRB1等位基因是某一人种或民族所特有的 ,但DRB1基因在不同种族的人群中有其各自独特的人群分布特征 ,并且在不同地域的同一民族人群中亦有其独特的地理分布特征。结论 :北方人在山东脐血库中最容易寻找到DR等位基因相合的异基因脐血供者。中国南方人和日本人在山东也完全可能寻找到DR等位基因相合的异基因脐血供者 ,其机率较北方人稍低。白种人甚至非裔美国人均有可能在山东脐血库中寻找到DR位点低分辨等位基因相匹配的异基因脐血造血干细胞。

The objective of the study was to research the distribution features of HLA-DRB1 alleles in Shandong Hans′ population and explore the possibility of finding the cord blood donor of HLA-DR matched to perform the stem cell transplantation for more patients from larger region of China and even other areas in the world. The subjects of the study were drawn from 3 438 Shandong Hans donors in Shandong Umbilical Cord Blood Bank and were tested by PCR-SSP technique for HLA-DR low resolution typing. The result indicated that the most prevalent five alleles of HLA-DRB1 locus were DRB1*15(0.1817),*07(0.1369),*09(0.1221),*04(0.1084)and *12(0.1038). The DR18 has the lowest gene frequency 0.0003,while DRB1*10,*16 and *01 showed lower gene frequencies (GF),which GF were 0.0151,0 0262,and 0.0322 respectively. As compared the HLA-DRB1 GF of Shandong Hans with those of other Han Chinese and other ethnic populations,there were unique distributed features of DRB1 alleles among various races populations,and those among the studied population groups from various regions with the same race origin. The difference from various regions in the same race was less than that among different races. In conclusion,a patient of Han Chinese is easier to search a DR-matched cord blood donor in Shandong Umbilical Cord Blood Bank,especially from northern Hans. No DRB1 allele is unique to single racial group and majority of DRB1 low-resolution phenotypes are common to all studied groups. It is reasonable for some patients from other races including Caucasian and Japanese to receive a transplant of cord blood stem cell matched with HLA-DR in Shandong Umbilical Cord Blood Bank.