脐血造血干细胞短期冻存效果的评价

Assessment on Effect of Short-Term Cryopreservation of Cord Blood Hematopoietic Cells

为了探讨脐血造血干细胞在液氮中短期冻存复苏后的效果 ,分别将各自为 8例冻存 6个月、1年、2年的脐血干细胞进行复苏 ,观察造血干细胞活性。有核细胞 (NC)计数采用自动血细胞分析仪测定 ,CD34+ 细胞采用流式细胞技术测定 ,用体外造血细胞培养技术分析粒 巨噬细胞集落生成单位 (CFU GM )产率 ,台盼蓝染色法判断造血细胞存活率。结果表明 :脐血造血干细胞在液氮中冻存 6个月、1年、2年后解冻 ,其有核细胞、CD34+ 细胞、细胞活率、CFU GM产率在 3个不同冻存时间的差异无显著性。结论 :脐血干细胞于液氮中短期冻存 ,其干细胞数量和细胞活性没有明显的变化 ,冻存效果较好。

To study the effects of short-term cryopreservation of cord blood hematapoietic cells in liquid nitrogen,the viability and function of cord blood hematapoietic cells were investigated by using each of 8 samples cryopreserved for six months,one and two years after thawing respectively. Nucleated cells (NC) were detected by blood cell analyzer. CD34+ cells were analyzed by flow cytometry, CFU-GM were cultured and detected in vitro,the survival rate was determined by trypan blue staining. The results showed that the differences of recovery rate of NC,CD34+,CFU-GM were nonsignificant at three different cryopreserved times. In conclusion,the short-term storage in liquid nitragen showed a good effect on cord blood hematapoietic cell without any significant change of activities and number of the cryopreserved hematapoietic cells.