摘要
采用四环素次抑菌浓度对嗜水气单胞菌进行选择 ,筛选得到耐四环素的嗜水气单胞菌菌株 ,其MIC(最低抑菌浓度 )为出发菌株的 8倍。通过比较对照菌与耐药菌总蛋白质的双向电泳图谱 ,获得 3个差异显著的蛋白点。对这 3个差异蛋白质进行肽质量指纹及其生物信息分析 ,分别鉴定为核糖体小亚基蛋白、药物排出系统蛋白和乙酸 乙酰辅酶A转移酶亚基。文中对嗜水气单胞菌耐四环素的机理进行了初步探讨。
In the article, the tetracycline-resistant Aeromonas hydrophlia was selected with antibiotic sub-inhibitory concentration of tetracycline. MIC of the resistant strains was eight times the original strains. To compare with 2-D PAGE of original strains, three proteins were achieved expressing conspicuous variances in resistant strains. By MALDI-TOF-MS and the Peptident software in the SWISS-PROT database, the variances were analyzed and elementarily identified as relevant with ribosomal small unit proteins, drug-discharged system proteins and acetate CoA-transferase beta subunit respectively. Besides, the resistant mechanism of Aeromonas hydrophlia was elementarily studied.
出处
《微生物学报》
CAS
CSCD
北大核心
2004年第3期396-398,共3页
Acta Microbiologica Sinica
基金
国家"8 63计划"( 2 0 0 2AA62 90 5 0 )
福建省自然科学基金 (B0 110 0 0 5 )
高等学校博士学科点专项科研基金 ( 2 0 0 10 3 840 0 9)~~
