用二苯代苦味酰基自由基分光光度法测定川芎、红景天和黄芪的抗氧化活性

Antioxidant activity detection of radix Ligusticum, radix Rhodiolae, and radix Astragali by using DPPH method

用乙醇提取川芎、红景天和黄芪三种中药中的抗氧化物质,并采用DPPH法测定各种中药提取液的抗氧化性,并同特丁基对苯二酚(TBHQ)、维生素E进行比较。结果表明,川芎提取液、红景天提取液和黄芪提取液对DPPH·有很好的清除作用,TBHQ、维生素E、川芎提取液、红景天提取液和黄芪提取液的IC50分别为5.2×10-6g/mL、4.5×10-3g/mL、4.5×10-4g/mL、7.8×10-5g/mL和3.1×10-3g/mL,其清除能力的大小依次为:TBHQ>红景天提取液>川芎提取液>黄芪提取液>维生素E。

Antioxidant substances of three kinds of Chinese traditional medicine were extracted with ethanol. Using the assay systems of DPPH, we determined their antioxidant activity and compared with TBHQ and Vitamin E. The result shows that the extracts of radix Ligusticum, radix Rhodiolae, and radix Astragali have good scavenging effects on DPPH. The antioxidant activity is TBHQ·>radix Rhodiolae>radix Ligusticum>radix Astragali>Vitamin E.