摘要
在真菌、昆虫、无脊椎动物和脊椎动物等许多分类单元中 ,都已发现mtDNA序列的核转座现象。在PCR扩增时 ,往往同时扩增出mtDNA和细胞核中线粒体假基因 (Numts) ,Numts混淆系统发育和群体遗传研究 ,得出错误的结果。本文综述了Numts的检查和避免的方法 ,以及在进化生物学研究中的应用进展。
The transposition of mtDNA sequences to the nucleus has been documented in a wide variety of taxa from fungi to insects to vertebrates. If co-amplified with mtDNA, Numts greatly complicate the accurate determination of mitochondrial sequences. The undiscovered presence of Numts could lead to incorrect results. Methods for checking and avoiding Numts have now been reviewed. However,several recent studies have demonstrated the potential utility of Numt DNA sequences serving as valuable molecular tools in evolutionary biology studies.
出处
《动物学杂志》
CAS
CSCD
北大核心
2004年第3期103-108,共6页
Chinese Journal of Zoology
基金
国家农业科技成果转化资金 (No .0 2EFN2 1 5 1 0 0 5 2 0 )资助
