小细胞肺癌组织c-kit蛋白表达的临床分析

Expression of c-kit protein and its significance in human small cell lung cancer

目的 探讨c kit蛋白在小细胞肺癌中的表达及其临床病理意义。方法 采用兔抗人c kit蛋白抗体、SP免疫组化方法检测 5 2例福尔马林固定、石蜡包埋的原发小细胞肺癌组织、10例正常肺组织和 2 0例非小细胞肺癌组织的c kit蛋白表达状况。结果 小细胞肺癌组织中c kit蛋白阳性表达率为 61.5 %( 3 2 /5 2 ) ,c kit蛋白表达定位于胞浆 ;其中强阳性者占 65 .6%( 2 1/3 2 ) ,弱阳性者占 3 4.4%( 11/3 2 )。 10例正常肺组织及 2 0例非小细胞肺癌组织c kit蛋白呈阴性表达。c kit蛋白表达与小细胞肺癌肿瘤大小 (P =0 .0 10 )及预后 (P =0 .0 0 0 1)有密切关系 ,但与性别、年龄及临床分期均无明显关系 (P >0 .0 5 )。结论 c kit蛋白表达可能与小细胞肺癌的预后相关。c kit蛋白的原位检测较为直观 ,有利于小细胞肺癌的临床病理诊断。

Objective To investigate the clinicopathological significance of c kit protein expression in small cell lung cancer (SCLC). Methods c kit protein expression was detected in 52 formalin fixed and paraffin embedded SCLC, 20 non small cell lung cancer (NSCLC) and 10 normal lung tissues by immunohistochemistry with rabbit antibody to human c kit protein. Results c kit protein was expressed in 61.5% (32/52) of primary SCLC, but negative in all 20 samples of NSCLC and 10 normal lung tissues. c kit protein was localized in cytoplasm of cancer cells. The expression of c kit protein was related to tumor size ( P =0.010) and prognosis ( P =0.000 1), but not to sex ( P =0.208), age ( P =1.000) or TNM stage ( P =0.722) of SCLC. Conclusion c kit protein expression may be corresponding to the prognosis of SCLC. Since in situ detection of c kit protein is more direct than the other methods, it is helpful to clinicopathological diagnosis, targeted therapies interacting with c kit signaling pathway and prognostic assessment in SCLC.