检测CK19 mRNA和MUC1 mRNA诊断非小细胞肺癌淋巴结微转移

Detection of mRNA expression of CK-19 and MUC1 gene for diagnosis of lymph node micrometastasis in NSCLC patients by reverse transcriptase-polymerase chain reaction

目的 探讨非小细胞肺癌 (NSCLC)淋巴结微转移的基因诊断方法 ,并分析CK19mRNA、MUC1mRNA作为肺癌微转移检测分子标志物的可行性。方法 应用巢式逆转录聚合酶链反应 (nestedRT PCR) ,对 3 1例NSCLC的 119枚淋巴结中的粘蛋白 1(MUC1)mRNA、角蛋白 19(CK19)mRNA表达情况进行检测 ;对照组为 10例肺良性病变患者的 3 5枚淋巴结。结果 3 1例NSCLC患者的 119枚淋巴结中 ,66枚 ( 5 5 .5 %)淋巴结存在CK19mRNA阳性表达 ,65枚 ( 5 4.5 %)存在MUC1mRNA阳性表达 ;肺良性病变患者 3 5枚淋巴结中CK19mRNA和MUC1mRNA表达均为阴性 ,与肺癌组比较均有显著性差异。结论 MUC1、CK19基因均可作为RT PCR法检测NSCLC患者淋巴结微转移的分子标志物 ,两者联合检测可能有助于早期诊断肺癌转移。

Objective To investigate gene diagnosis of micrometastasis in lymph nodes in patients with non small cell lung cancer (NSCLC) and the feasibility of mucin 1 (MUC1) mRNA and cytokeratin 19 (CK19) mRNA as molecular marker to detect micrometastasis of lung cancer. Methods Expression of MUC1 mRNA and CK19 mRNA was detected in 119 lymph nodes taken from 31 patients with NSCLC, 35 lymph nodes from 10 patients with pulmonary benign diseases as controls by nested reverse transcriptase polymerase chain reaction (RT PCR). Results In the 119 lymph nodes from lung cancer patients, CK19 mRNA expression was detected in 66 lymph nodes (55.5%) and MUC1 mRNA expression was detected in 65 lymph nodes (54.5%) by RT PCR. Neither CK19 mRNA nor MUC1 mRNA expression was observed in all the 35 lymph nodes in the benign pulmonary lesion group. Conclusion The results suggest that the detection of both MUC1 and CK19 mRNA might be helpful to diagnose NSCLC micrometastasis in lymph nodes. The establishment of this method may lead to an earlier diagnosis of metastasis for lung cancer.