检测肺癌中辅助性T细胞(Th_1/Th_2)的临床意义

The clinical significance of detection of Th_1/Th_2 cell cytokines in lung cancer

目的 探讨辅助性T细胞Th1 和Th2 细胞因子对肺癌患者的临床意义 ,为肿瘤的免疫治疗提供依据。方法 采用放射免疫 (RIA)和酶联免疫吸附法 (ELISA)检测 86例肺癌患者、5 9例肺良性病患者及45例正常对照辅助性T细胞分泌的细胞因子。以IL 2和TNF α的水平代表Th1 型细胞因子 ,IL 4、IL 6和IL 8的水平代表Th2 型细胞因子。结果 肺癌患者IL 2 [( 2 4.6± 12 .0 ) μg/L]的水平显著低于肺良性病患者[( 71.1± 2 5 .4) μg/L] (t =3 .82 ,P <0 .0 1)和正常对照 [( 69.3± 19.5 ) μg/L] (t =2 .76,P <0 .0 1) ,IL 6[( 0 .13± 0 .0 4) μg/L]的水平显著低于正常对照 [( 0 .2 3± 0 .0 5 ) μg/L ) (t =3 .3 9,P <0 .0 1) ,IL 4[( 2 5 4.2±78.0 ) μg/L]、IL 8[( 0 .49± 0 .16) μg/L]、TNF α[( 2 .76± 1.12 ) μg/L]的水平明显高于肺良性病患者 [( 63 .6± 18.6) μg/L ,( 0 .3 6± 0 .18) μg/L ,( 0 .96± 0 .2 0 ) μg/L]及正常对照 [( 60 .9± 19.6) μg/L ,( 0 .3 5± 0 .0 7) μg/L ,( 0 .93± 0 .19) μg/L] (t值分别为 4.10、4.89和 3 .76,P均 <0 .0 1) ,肺良性病患者和正常对照之间的IL 2、TNF α、IL 4、IL 8均未见明显差异 (P >0 .0 5 ) ,肺癌组IL 6的水平与肺良性病组 [( 0 .15± 0 .0 4)

Objective To explore the clinical significance of detection of T helper cell (Th 1 and Th 2) in patients with lung cancer and to provide a foundation for immunological treatment. Methods RIA and ELISA were used to detect the level of serum IL 2, IL 4, IL 6, IL 8 and TNF a in 86 patients with lung cancer, 59 patients with benign pulmonary diseases and 45 healthy people. IL 2 and TNF a were used to represent cytokines of Th 1 type, and IL 4, IL 6 and IL 8 to represent cytokines of Th 2 type. Results The level of IL 2 [(24.6±12.0) μg/L]in cancer group was significantly lower than that in benign group [(71.1±25.4) μg/L] ( t =3.82, P <0.01) and normal group [(69.3±19.5) μg/L]( t=2.76, P <0.01), the level of IL 6 in cancer group [(0.13±0.04) μg/L] was significantly lower than that in normal group [(0.23±0.05) μg/L]( t= 3.39 , P <0.01), but the levels of IL 4 [(254.2±78.0) μg/L], IL 8 [(0.49±0.16) μg/L], and TNF a [( 2.76 ±1.12) μg/L] in cancer group were significantly higher than those in benign group [(63.6±18.6) μg/L, ( 0.36 ±0.18) μg/L, (0.96±0.20) μg/L respectively] and those in normal group [(60.9±19.6) μg/L, ( 0.35 ±0.07) μg/L, (0.93±0.19) μg/L respectively] ( t =4.10, 4.89, 3.76 respectively, all P <0.01). No significant difference of IL 2, IL 4, IL 8 and TNF a level was observed between benign group and normal group (all P >0.05). The level of IL 6 in cancer group was similar to that in benign group [(0.15±0.04) μg/L] ( P > 0.05 ). The level of IL 6 in benign group was significantly lower than that in normal group [(0.23±0.05) μg/L] ( P > 0.05 ). There was no significant difference in these cytokines among lung cancer patients with different histological types and in different TNM stages.Conclusion T helper cell cytokines are out of balance in patients with lung cancer, and this may play a certain role in the pathogenesis of lung cancer. Correcting this immune malfunction may become an important method in lung cancer therapy.