G0101C03-3和L0254H10-3基因在小鼠动情周期子宫中的差异表达

Differential expression of G0101C03-3 and L0254H10-3 in mouse uterus during the estrous cycle

正常动情周期的维持是小鼠子宫多功能的必要条件 ,但对于其分子基础至今尚不十分明了。我们曾通过基因芯片技术分析了小鼠动情期与间情期子宫的基因表达谱 ,发现了许多差异表达的基因或表达序列标签(ESTs)。本实验选取了G0 1 0 1C0 3 3及L0 2 5 4H1 0 3两个差异表达的EST ,通过Northern印迹与原位杂交方法分析了它们在小鼠动情周期子宫中的时空表达模式。结果表明 :这两个基因的表达水平都发生周期性变化 ,在动情期表达量较少 ,而在间情期表达量较高 ;G0 1 0 1C0 3 3在动情期的表达量是间情期的 2 7% ,L0 2 5 4H1 0 3在动情期检测不到有表达 ,提示它们的表达受卵巢类固醇激素的调控 ;G0 1 0 1C0 3 3基因主要在子宫的腔上皮与腺上皮中表达 ,可能与子宫细胞的程序性死亡有关 ;而L0 2 5 4H1 0

Temporal and spatial change of two differential express sequence tags (EST), G0101C03 3 and L0254H10 3 , in cycling mice uteri were studied by Northern blot analysis and in situ hybridization. It was demonstrated that the expression level of G0101C03 3 and L0254H10 3 was higher at the diestrous stage and much lower at the estrous stage. The expression of G0101C03 3 at the estrous stage was 27% of the expression at the diestrous stage. The expression of L0254H10 3 at the estrous stage could not be detected. In situ hybridization further showed that the transcript of G0101C03 3 was mainly localized in the luminal and glandular epithelium during the estrous cycle, whereas L0254H10 3 in uterine stromal cells. The data indicated that the genes might participate in some cell events such as apoptosis in mice uteri under the regulation of ovarian steroid hormones.