一个陆地棉bZIP蛋白cDNA的克隆及表达分析(英文)

Cloning and Expression Analysis of a bZIP cDNA in Gossypium hirsutum L.

利用PCR筛选方法从陆地棉纤维cDNA文库中筛选到一个全长cDNA序列 ,命名为GhbZIP。其编码产物长度为 6 4 5个氨基酸残基 ,序列中含有两个未知功能的保守区域DUF6 30和DUF6 32 ,而DUF6 32区中有一个类似碱性亮氨酸拉链基元 ;此外氨基酸序列中还存在一个富脯氨酸区和一个富苯丙氨酸区 ,因此该蛋白具有植物碱性亮氨酸拉链蛋白的结构特征。亲水性分析表明 ,GhbZIP为一个典型的膜蛋白。GhbZIP基因主要是在开花 3d之后在胚珠和纤维细胞中表达 ,这表明该基因可能与棉纤维伸长过程中的基因表达调控有关。

A new full-length cDNA clone was isolated from the fiber cDNA library of Gossypium hirsutum L.The cDNA,designated GhbZIP,encoded a polypeptide of 645 amino acids.GhbZIP protein had the structure characteristics of plant (bZIP) proteins,including two conserved domains,DUF630 and DUF632 with unknown functions,a proline-rich domain and a phenylalanine-rich domain.Meanwhile,the protein contained a leucine zipper-like motif in DUF632.The hydropathy analysis showed that the GhbZIP was a membrane protein.GhbZIP gene was preferentially expressed in ovule and fiber cells since three days post-anthesis,as indicated it might be involved in the transcription regulation of genes during cotton fiber elongation.