脊髓半切伤大鼠血清谷丙转氨酶和谷草转氨酶活性变化的研究

Serum activities of alanine aminotransferase and aspartate aminotransferase in rats with hemi sected spinal cord injury

目的:探讨大鼠血清谷丙转氨酶(ALT)和谷草转氨酶(AST)的活性变化与脊髓半切伤严重程度的关系。方法:测定30只大鼠脊髓半切伤急性期和恢复期血清中ALT和AST的水平以及10只对照组大鼠血清的ALT和AST的浓度。结果:脊髓半切伤大鼠急性期血清中ALT,AST活性分别为(51.01±11.08)和(56.08±20.12)nkat/L明显高于假手术组急性期活性犤(36.01±8.79),(30.57±12.98)nkat/L,t=1.32,0.56,P<0.01犦。脊髓半切伤大鼠急性期比恢复期血清中ALT和AST活性犤48.05±18.06),(51.09±11.08)nkat/L犦明显升高。结论:ALT,AST在脊髓半切伤急性期呈反应性升高,与脊髓损伤严重程度呈正相关。

AIM:To explore the relationship between the activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) and the severitiy of hemi sected spinal cord injury (hSCI) in rats. METHODS:ALT and AST activities in serum of 30 hSCI rats at acute and recovery stage, as well as those of 10 rats in controlled group,were statistically analyzed respectively. RESULTS:The activities of ALT and AST in serum of acute hSCI rats was (51.01±11.08) and(56.08±20.12) nkat/L respectively,which was obviously higher than those in sham operation group[(36.01±8.79), (30.57±12.98) nkat/L,t=1.32, 0.56,P< 0.01].The activities of ALT and AST in serum of hSCI rats at acute stage significantly increased as compared with those at recovery stage [(48.05±18.06),(51.09±11.08)nkat/L]. CONCLUSION:The activities of ALT and AST at acute stage of hSCI reactively improves, and positively relates to the spinal cord injury severity.