东莨菪碱和氯胺酮预防主动脉阻断所致脊髓缺血性损伤(英文)

Prevention of rabbit ischemic spinal cord injury following aortic cross clamping using scopolamine and ketamine

目的:评价东莨菪碱和氯胺酮对主动脉阻断所致脊髓缺血性损伤保护作用。方法:28只新西兰大白兔随机数字表法分成对照组、东莨菪碱组、氯胺酮组、东莨菪碱+氯胺酮组。每组肾下主动脉阻断30min,动态监测脊髓血流量(SCBF)、后肢运动功能、组织水含量及组织学改变。结果:再灌注20h,后肢运动功能东莨菪碱+氯胺酮组优于对照组(t=5.36,P<0.05),脊髓水含量东莨菪碱+氯胺酮组比对照组减少10.5%(t=4.01,P<0.01),组织学改变东莨菪碱+氯胺酮组最轻,对照组最严重。阻断中及开放2h东莨菪碱+氯胺酮组SCBF比值较对照组显著增加。结论:东莨菪碱和氯胺酮联合应用比单独应用能更有效地预防脊髓缺血性损伤。

AIM:To evaluate the protective effects of scopolamine and ketamine on spinal cord injury following cross clamping of rabbit aorta. METHODS:Twenty eight New Zealand rabbits were randomly divided into 4 equal groups,namely the control,scopolamine(S),ketamine(K) and scopolamine+ketamine(S+K) groups.The infrarenal aorta of all rabbits was occluded for 30 minutes followed by observations of the spinal cord blood flow(SCBF),hinder limb motor function,tissue water content and histopathological changes. RESULTS:Twenty hours after reperfusion,the hinder limb motor function was better in S+K group than in the control group(P< 0.05),and the spinal cord water content was 10.5%higher in the control group(8.4%±6.9%) than in the S+K group(69.9%±0.6%,P< 0.01). Histopathological changes were the slightest in S+K group and the most serious in the control group.The ratio of SCBF in S+K group significantly increased compared with that in the control group(P< 0.01) during clamping of the aorta and 2 hours after the ischemia. CONCLUSION:Scopolamine in combination with ketamine is more effective in preventing spinal cord ischemic injury than either of the agents used alone.