金安复方中药制剂对Lewis肺癌小鼠抑瘤及增加体质量的机制(英文)

Mechanism of tumor inhibition and body mass increase of Chinese traditional medicine fufang jin'an therapy in mice with Lewis lung cancer

目的:探讨中药金安对C57BL/6J小鼠Lewis肺癌的抑制效应及其作用机制。方法:荷Lewis肺癌的C57BL/6J小鼠分为生理盐水组、金安高、中、低剂量组和环磷酰胺组,通过检测各组小鼠的体质量变化和抑瘤率,应用流式细胞仪和TUNEL等方法观察金安对Lewis肺癌的抑瘤机制。结果:金安高、中、低剂量组体质量均明显增加,且金安高、中、低剂量组与环磷酰胺组的抑瘤率依次为45.79%,40.90%,32.48%和98.96%,流式细胞仪检测金安各组肿瘤细胞凋亡率分别为24.19%,14.95%和13.93%,并经统计学分析发现金安诱导肿瘤细胞的凋亡率与剂量之间存在依赖关系。TUNEL检测各组凋亡指数(AI)依次为0.79±1.60,6.43±5.62,4.00±7.15,1.14±1.90,经方差分析知金安高剂量组与NS组差异有显著性意义。结论:金安对Lewis肺癌生长具有一定的抑制作用,且促进肿瘤细胞凋亡可能是金安治疗Lewis肺癌的主要作用方式。

AIM:To investigate the inhibitive effects and mechanism of action of Chinese herb Jin'an in C57BL/6J mice with Lewis lung cancer(LLC). METHODS:C57BL/6J mice bearing LLC were divided into normal saline(NS) group,high dose Jin'an group,medial dose Jin'an group,low dose Jin'an group and cytoxan(CTX) group.Flow cytometry and TUNEL method were used to observe the mechanism of tumor inhibition of Jin'an in LLC by means of detecting tumor inhibition rates and body mass changes of the mice in each group. RESULTS:The body mass of high dose,medial dose and low dose Jin'an groups were obviously increased,and the tumor inhibition rates of high dose,medial dose,low dose Jin'an groups and CTX group were 45.79%,40.90%,32.48%and 98.96%respectively.The apoptotic rates of tumor cells in each Jin'an group were 24.19%,14.95%and 13.93%respectively,which were detected with flow cytometry and correlative relationship was found between the apoptotic rates of tumor cells induced by Jin'an and the dosage through statistical analysis.The apoptosis indexes(AI) of each group were 0.79±1.60,6.43±5.62,4.00±7.15,1.14±1.90 respectively,and there was significant difference between high dose Jin'an group and NS group by analysis of variance. CONCLUSION:Jin'an has certain inhibitive effects on the growth of LLC and Jin'an can also promote apoptosis of tumor cells,which is probably the main action of Jin'an treatment in LLC.