多效唑对马铃薯试管苗生长和块茎形成的影响

Effect of paclobutrazol on explant growth and tuberization in potato.

采用离体培养方法,研究了不同浓度多效唑处理对马铃薯试管苗生长和块茎形成的影响.结果表明,0.1～1mg/L多效唑处理完全抑制了MS扩繁培养基中马铃薯试管苗的生长,而0.01mg/L多效唑处理虽抑制了试管苗茎叶的生长,但叶色加深、根重和根长显著增加.在试管薯诱导条件下,0.1mg/L多效唑处理促进了'夏波蒂'提早结薯,使单瓶试管薯鲜重、平均直径和单薯鲜重都显著增加,同时降低了畸形薯率;而单瓶试管薯的结薯数量低于BA+CCC处理,但接近对照.这些结果表明:与广泛应用的矮壮素相比,在马铃薯试管薯生产中使用多效唑能提高试管薯产量和质量,且其使用浓度仅为矮壮素的1/5000,有利于降低残留并节约成本,是矮壮素较好的替代物之一.

The growth of potato explants was inhibited completely by the application of 0.1-1 mg/L paclobutrazol in the MS propagation medium. The growth of stems and leaves was inhibited, but leaf color was more green and root fresh weight and root length were significantly increased by 0.01 mg/L paclobutrazol treatment. Under the inductive condition, 0.1 mg/L paclobutrazol treatment promoted the earlier microtuber formation, increased microtuber fresh weight per bottle, mean diameter and mean fresh weight per microtuber, and reduced the percentage of abnormal microtubers, although the microtuber number per bottle was lower than that of BA+CCC (chlorocholine chloride) treatment (but close to the control). These results indicated that paclobutrazol could effectively promote the yield and quality of potato microtubers, and its suitable concentration was only 1/5000 of CCC which was applied widely. Thus, paclobutrazol appears suitable for routine use in potato in vitro tuberization media and may have more advantages than CCC.