可德兰多糖的分离纯化和化学结构分析

Study on separation, purification and chemical structure of Curdlan polysaccharide.

用酸碱法从产碱杆菌突变株C125的发酵液中提取Curdlan粗多糖样品CD-1,经Sevage法脱蛋白、纤维素柱层析脱色后,用SephadexG-100凝胶层析柱分离纯化得多糖CD-2.紫外分光法及SephadexG-200凝胶柱层析法检测CD-2为均一多糖,SephadexG-200柱层析测得CD-2的分子量为65300Da,完全酸水解后纸层析检测CD-2的单糖组成中仅有葡萄糖,分析CD-2的红外光谱、核磁共振谱得知CD-2多糖的化学结构是以β-(1,3)糖苷键连接而成的无分枝的葡聚糖.

Curdlan polysaccharide was isolated from the fermentation broth of Alcaligenes faecelis var.C125 by acid and alkali and the rude sample CD-1 was gained. The protein and pigment were removed from CD-1 by Sevage method and cellulose column chromatography, respectively. The pure sample CD-2 was separated by Sephadex G-100 column chromatography. The CD-2 was a homogeneous pure polysaccharide confirmed by UV-spectrometer and Sephadex G-200 chromatography, and its molecular weight determined by Sephtrometer-200 column chromatography was 65300D. CD-2 was composed of only glucose, which was proved by paper chromatography analysis after complete hydrolysis of the pure sample by sulfuric acid . And the chemical structure of CD-2 was indicated as a kind of linear glucan linked by β-(1,3) linkage by infrared spectroscopy and ()^(13)C-NMR.