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应用实时定量PCR技术研究人重组干扰素α2b在人群中对麻疹病毒和风疹病毒的抑制作用 被引量：3

Studies of the Effect of Interferon α2b on Inhibition of Measles Virus and Rubella Virus Replication by Using Real-time PCR Method
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摘要 Human recombinant interferon α2b can inhibit replication of many viruses and it is an important prophylactic drug for virus infection. In this study, interferon α2b was used to study the biological function on measles virus and rubella virus replication in human. For each virus, selected population was divided into two groups, including with and without interferon α2b treatment, then the attenuated viral vaccine was used to inoculate each group and the respiratory samples were collected at fixed intervals. A highly sensitive and accurate Real-time PCR method was used to detect the virus copy number in samples. The results showed that the virus copy number was much lower after interferon treatment and suggested that interferon α2b could inhibit the measles virus and rubella virus replication in human. Human recombinant interferon α2b can inhibit replication of many viruses and it is an important prophylactic drug for virus infection. In this study, interferon α2b was used to study the biological function on measles virus and rubella virus replication in human. For each virus, selected population was divided into two groups, including with and without interferon α2b treatment, then the attenuated viral vaccine was used to inoculate each group and the respiratory samples were collected at fixed intervals. A highly sensitive and accurate Real-time PCR method was used to detect the virus copy number in samples. The results showed that the virus copy number was much lower after interferon treatment and suggested that interferon α2b could inhibit the measles virus and rubella virus replication in human.
作者 舒跃龙 周蕊 刘丽琦 史智扬 朱凤才 赵静 李刚 唐震 林里卓 张丽兰 侯云德
机构地区 病毒生物技术国家工程研究中心 江苏省疾病预防控制中心 徐州市铜山县疾病预防控制中心 北京远策药业有限责任公司
出处 《病毒学报》 CAS CSCD 北大核心 2004年第2期166-168,共3页 Chinese Journal of Virology
关键词 实时定量PCR 干扰素 人群 麻疹 副粘病毒科 麻疹病毒 interferon α2b Real-time PCR measles virus rubella virus
分类号 R978.7 [医药卫生—药品] Q78 [生物学—分子生物学]
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参考文献9

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二级参考文献32

  • 1张丽兰,吴德华,侯云德.黄芪对基因工程人α2b型干扰素抗鼻病毒14型的协同作用[J].中华实验和临床病毒学杂志,1996,10(1):77-80. 被引量：6
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共引文献28

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二级引证文献13

病毒学报
2004年 第2期

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