松果菊苷抗衰老作用机理研究

STUDY ON MOLECULAR MECHANISM OF ECHINACOSIDE FOR AGAINST AGING

研究了中国传统药物肉苁蓉提取物松果菊苷(echinacoside,ECH)体外清除活性氧自由基和体内抗氧化、抗衰老作用的机理。运用电子顺磁共振(electronparamagneticresonance,EPR)自旋捕捉方法研究ECH对体外产生的羟自由基(·OH)、超氧阴离子自由基(O2·)和脂自由基(L·)的清除能力;并以D-半乳糖衰老小鼠为实验模型,采用低温EPR技术直接检测小鼠心、肝、肾、脑组织活性氧物种(reactiveoxygenspecies,ROS)水平;生化方法检测小鼠全血谷胱甘肽过氧化物酶(glutathioneperoxidase,GSH-Px)和脑组织单胺氧化酶(monoaminoxidase,MAO)活性及肝组织丙二醛(malondialdehyde,MDA)含量;EPR自旋捕捉方法检测血清超氧化物歧化酶(superoxidedismutase,SOD)活力;跳台法检测小鼠记忆力。结果表明ECH能较好抑制体外·OH、O2·和L·自由基,同时能够提高GSH-Px和SOD活性,降低MDA含量,因此对D-半乳糖所致衰老引起的活性氧自由基损伤具有一定修复作用。由于抑制了MAO活性而提高小鼠的记忆力。由此可以认为ECH抗脂质过氧化及改善衰老的作用与其抗氧化活性有关。

It was investigated that the scavenging abilities of Echinacoside (ECH) to reactive oxygen species (ROS) in vitro and in vivo,as well as the molecular mechanism for anti-aging by ECH. The inhibitory effect of ECH on ·OH?O2· and L· free radicals in vitro was evaluated with spin trapping EPR method. Cryogenic EPR technique was used to detect the generation of reactive oxygen species (ROS) in heart, liver, kidney and brain of an aged mice induced by D-galactose (D-gal). The content of MDA in liver homogenate, the activities of MAO in brain homogenate, and whole blood GSH-Px were determined by biochemistry method; The activity of SOD in serum was calculated from spin trapping EPR spectra. The memory potency of mouse was checked using step down test. The results showed that ECH possessed a good scavenging potency on ·OH?O2· and L· free radicals in vitro, they could repair the damages induced by aging, as well as enhance the activities of GSH-PX and SOD, reduce the content of MDA. ECH could improve the memory ability from decreasing the activity of MAO. It was concluded that ECH might delay aging process and inhibit the lipid peroxidation, which related to their antioxidative function.