野生来源TW小鼠近交系培育及其生物学特性的研究

Establishment of TW Inbred Strain Derived from Wild Mouse and Studies on Its Biological Characteristics

目的 培育中国特有TW (TianjinWild)野生小鼠来源的实验近交小鼠品系 ,筛选其重要的生物学特征 ,丰富现有实验动物基因库。方法 严格按照国际培育近交系动物标准 ,即每代繁育的雌雄小鼠严格按照同胎雌雄 (兄妹 )进行交配。结果 经过 1 2年的精心培育 ,培育TW小鼠至F2 2代 ,其他 1 5地野生小鼠品系均因故中断。结论 该项研究将为丰富国际现有实验小鼠基因库资源 ,建立抗肺肿瘤特性实验小鼠模型动物奠定坚实基础。

Objective To breed and establish a new wild derived inbred strain of mice trapped in China, and select its important biological characteristics for enriching present gene bank of laboratory animals. Methods Wild mice were inbred strictly according to the international standards, that is, brother sister cross breeding by the same litters. Results Through 12 years of inbreeding, TW (Tianjin Wild) mice were inbred to F22 generation. And mouse strains from other 15 trapping localities were all interrupted or stopped by genetic reasons. Conclusion This study may enrich international gene bank of laboratory mouse and lay a foundation for establishing the laboratory mouse model of anti-lung tumors.