蛋白激酶Cγ和蛋白磷酸酯酶Aα在慢性复合应激性学习记忆增强大鼠海马中表达的变化

EXPRESSION OF PKCγ AND PP2B-Aα IN THE HIPPOCAMPUS OF RATS WITH LEARNING AND MEMORY ENHANCEMENT BY CHRONIC COMPLEX STRESS

目的 探讨蛋白激酶Cγ (PKCγ)和蛋白磷酸酯酶Aα (PP2B Aα)在慢性复合应激性学习记忆增强大鼠海马中的表达及其意义。方法 将成年雄性Wistar大鼠随机分为应激组和对照组 ,对应激组实行慢性复合应激 4 2d后 ,用Morris水迷宫 ,Y 迷宫对 2组大鼠进行学习和记忆能力测试 ,然后用免疫组织化学方法 ,观察 2组大鼠PKCγ和PP2B Aα的表达 ,并用计算机图像分析系统对免疫阳性反应结果进行处理。结果 应激组比对照组学习记忆能力明显增强 ;在海马CA3区PKCγ的免疫反应阳性明显增强 ;PP2B Aα在海马CA1和CA3区的免疫反应阳性明显减弱。结论 海马内PKCγ和PP2B Aα表达的变化可能参与了慢性复合应激致大鼠学习记忆增强的机制。

Objective To study the expressions and significance of PKCγ and PP2B-Aα in the hippocampus of rats with chronic complex stress-induced learning and memory enhancement.Method Adult male Wistar rats were randomly divided into stress and control groups. After the stressed rats were exposed to chronic complex stress 6 h per day for 42 days, the learning and memory ability of the two groups were tested respectively by using the morris water maze and Y-maze. The expressions of PKCγ and PP2B-Aα in the hippocampus were observed by the immunohistochemical method, and the results were analyzed by the computer image analysis system. Result The ability of spatial learning and memory of the stressed rats was enhanced compared with that of the controls the immunoreactivity of PKCγ in CA3 region of the hippocampus of the stressed rats was increased, while that of PP2B-Aα was decreased in both CA1 and CA3 areas of the hippocampus in the stressed rats. Conclusion Alteration in the expressions of PKCγ and PP2B-Aα in the hippocampusmay participate in the chronic complex stress-induced spatial learning and memory enhance ment of rats.