期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNA干涉技术 被引量：1

RNA interference
下载PDF
导出
摘要 RNA干涉(RNAi)技术是利用一些小的双链RNA来高效、特异地阻断体内特定基因的表达,并促使mRNA降解,从而诱使细胞表现出特定基因缺失的表型。本文从RNAi技术的历史、作用机制、研究策略、研究现状及应用前景等几个方面进行了综述,预测RNAi将会给基因治疗的发展带来新的希望。 Small interfering RNAs(siRNAs)are short,double-stranded RNA molecules that can target complementary mRNAs for degradation via a cellular process termed RNA interference(RNAi).In this paper,the history and mechanism of RNAi as well as the method and advance of RNAi research were summarized.
作者 胡迎春 李刚 吴德昌
机构地区 军事医学科学院放射医学研究所
出处 《生物技术通讯》 CAS 2004年第3期285-288,共4页 Letters in Biotechnology
关键词 RNA干涉 RNAI 基因 表达 双链RNA 基因沉默 RNA interference double-stranded RNA gene silencing
分类号 Q522 [生物学—生物化学] Q78 [生物学—分子生物学]
  • 相关文献

参考文献29

  • 1段发平,梁承邺.转录后水平沉默与基因表达[J].生物学通报,2002,37(3):15-16. 被引量：10
  • 2Oh WJ, Kim EK, Ko JH, et al. Nuclear proteis that bind to metal response element a(MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene [J]. Eur J Biochem, 2002,269(8):2151
  • 3Jorgensen RA, Cluster PD, English J, et al. Chalcone synthase cosuppres sion phenotypes in petunia flowers:comparison of sense vs.antisense con structs and single-copy vs.complex T-DNA sequences [J]. Plant Mol Biol,1996,31:957
  • 4Ketting RF, Haverkamp TH, Van Luenen HG, et al. Mut-7 of C. elegans,required for transposon silencing and RNA interference,is a homolog of Werner syndrome helicase and RNase D[J]. Cell, 1999,99:133
  • 5Miyagishi M, Taira K. U6 promoter-driven siRNAs with four uridine 3' overhangs efficiently supperss targeted gene expression in mammalian cells [J]. Nature Biotechnol, 2002,19:497
  • 6Harborth J, Elbashir SM, Bechert K, et al. Identification of essentiall gene in cultured mammalian cells using small interfereing RNAs [J]. J Cell Science, 2001,114(24):4557
  • 7Hammond SM,Caudy AA,Hanmon GJ, et al. Post-transcriptional gene si lencing by double-stranded RNA[J]. Nature Rev Gen, 2001,2:110
  • 8Bernstein E, Caudy AA, Hammond SA, et al. Role for a bidentate ribonu clease in the initiation step of RNA interference[J]. Nature, 2001,409:363
  • 9Nykanen A, Haley B, Zamore PD. ATP requirements and small interfering RNA structure in the RNA interference pathway[J]. Cell, 2001,107:309
  • 10Lipardi C, Wei Q, Paterson BM. RNAi as random degradative PCR. siRNA primers convert mRNA into dsRNA that are degraded to generate new siR NAs[J]. Cell, 2001,107:297

二级参考文献6

  • 1Hammond SM, Caudy AA, Hannon, GJ. Posttranscriptional gene silencing by double-stranded RNA.Nat Rev Genet. 2001,2(2) : 110-119.
  • 2Carthew RW. Gene silencing by double-stranded RNA.Curr Opin Cell Biol. 2001,13(2) :244-8.
  • 3Finnegan E J, Wang M, Waterhouse P. Gene silencing:Fleshing out the bones. Curr Biol. 2001,11 (3): 99-102.
  • 4Gura T. A silence that speaks volumes. Nature 2000,404:804-808.
  • 5Hamilton AJ,Baulcombe DC. A species of small antisense RNA in posttranscriptional gene silencing in plants. Science, 1999,286: 950-952.
  • 6Baulcombe D. Viruses and gene silencing in plants. Arch Virol Suppl, 1999,15 : 189-201.

共引文献9

同被引文献3

引证文献1

二级引证文献4

生物技术通讯
2004年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部