组织工程用真皮成纤维细胞渗透特性的初步研究

A Primary Study on the Water Osmotic Characteristics of Fibroblasts Employed in Tissue Engineered Dermal Replacement

在优化组织工程化真度低温保存程序时,需要了解体外培养的真皮成纤维细胞的渗透特性。利用细胞计数与尺寸分析仪初步研究了组织工程用真皮成纤维细胞对水的渗透特性,其中包括细胞的等渗体积、细胞在低渗或高渗溶液(渗透压范围:130～1250 mOsm)中细胞的平衡体积及细胞的不可渗体积。结果表明,在等渗条件下(280.67 mOsm),真皮成纤维细胞的平均体积为5105.51μm^3(直径d=21.40μm);细胞体积随溶液渗透压的变化规律符合Boyle van’t Hoff关系式,据此得到真皮成纤维细胞的不可渗体积V_b=0.3631V_i,为1853.81μm^3。

To predict optimal protocols for cryopreservation of tissue engineered dermal equivalent, knowledge of fundamental cell water osmotic characteristics is required. In this study, the osmotic characteristics of cultured dermal fibroblasts were investigated using Coulter particle count and size analyzer. The normal cell volume is 5105.51μm^3(d=21.40μm)measured at initial (isotonic) osmolality, 280.67 mOsm. The cell volume changes were measured after the cells were exposed and equilibrated to different anisosmotic conditions. The cell volume was found to be a linear function of the reciprocal of the extracellular osmolality (Boyle van't Hoff plot) ranging from 130 to 1250 mOsm. The non-osmotic volume of the dermal fibroblast is 0.36 of the cells isotonic volume.