摘要
采用正交设计方法对影响芥蓝植株再生体系的因素进行了优化研究,结果表明:影响芥蓝植株再生的最主要因素是外植体类型,其次依次为NAA,BAP,蔗糖和AgNO_3。结果进一步显示,最利于芥蓝再生植株的培养基条件为:MS+BA P 2 mg/L+NAA 0.03 mg/L+1％蔗糖+AgNO_37.0 mg/L+0.8％琼脂,最适宜的外植体类型为下胚轴,植株再生频率高达97.5％。
By the orthogonalty design, the factors which influenced the regeneration of Brassica oleracea L.
var. alboglabra were studied, the results showed that the major factor is the explant, and the next are NAA, BAP,
sucrose and AgNO_3, the best recipe of the culture medium for the regeneration is: MS+NAA 0.03 mg/L+BAP 2mg/L+1%
sucrose+AgNO_37.0 mg/L+0.8% agar. The optimum explant is the hypocotyls with the regeneraion frequency 97.5%.
出处
《细胞生物学杂志》
CSCD
北大核心
2004年第3期313-316,共4页
Chinese Journal of Cell Biology
基金
浙江省重大科技项目(021102536)资助
关键词
芥蓝
再生体系
正交设计
外植体
培养基
Brassica oleracea
Chinese broccoli
regeneration
orthogonalty design
