NAA和IBA对四倍体刺槐试管苗生根影响及不定根发育过程解剖观察

Effect of NAA and IBA on Rooting of Tetraploid Robinia pseudoacacia in vitro and Anatomical Observation on Adventitious Root Development

对四倍体刺槐无性系试管苗不定根的诱导进行了研究。采用 32 析因设计、二次回归正交设计的方法研究了在培养基中添加植物生长调节剂NAA和IBA对试管苗生根的影响 ,通过统计分析选出四倍体宽叶刺槐无性系的最适生根培养基是 1 2MS +IBA 0 4mg·L- 1 +NAA 0 2 5mg·L- 1 +蔗糖 2 % +琼脂 0 6 % ;并对四倍体刺槐无性系试管苗不定根的发育过程进行了解剖观察 ,结果表明试管苗嫩梢无潜伏根原基 ,不定根由诱生根原基发育形成 ,诱生根原基源于髓射线细胞的分裂和分化。

The effect of NAA, IBA on rooting of shoots in test tubes of the tetraploid Robinia pseudoacacia was studied by the aid of the different test designs such as factorial experiment, regressive orthogonality test. The optimal medium was 1/2 MSIBA 0.4 mg·L -1NAA 0.25 mg·L -1sucrose 2%agar 0.6%. The adventitious root formation of shoots in test tube was also observed by means of the anatomical method. Latent root primordium was not found in shoots in test tubes. The induced root primordium which originated from the division and differentiation of pith ray cell developed into the adventitious roots.