乐陵无核小枣组培快繁影响因素研究

Favorable conditions for tissue culture of Chinese date variety 'Lelingwuhe'

以乐陵无核小枣试管苗为材料,对其组培快繁过程中的影响因素进行了研究。结果表明,乐陵无核金丝小枣继代快繁的适宜培养基和培养条件是:改良MS培养基+BA2.0mg/L+NAA0.2mg/L,培养温度28℃,光照时间16小时/天,光照强度3000lx,pH值6.1,蔗糖浓度30g/L,琼脂用量7g/L。

Studies revealed that the favorable conditions for subculture of the stoneless jujube 'Lelingwuhe' were the improved MS medium(pH 6.1,sucrose 30g/L,agar 7g/L)+BA 2.0mg/L+NAA 0.2mg/L,culture temparature 28℃,illumination 16h/d at 3000lx.