期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

绿色荧光蛋白在胚胎干细胞研究中的应用 被引量：3

Applications of Green Fluorescent Protein (GFP) in the Research of Embryonic Stem Cells
下载PDF
导出
摘要 绿色荧光蛋白(green fluorescent protein,GFP)作为一种方便、有效的活性标记物,无需外源反应底物,无需进行细胞或组织的固定和渗透处理,使检测更加方便。是细胞生物学和分子生物学研究的一个重要工具,已得到了广泛的应用。胚胎干细胞(Embryonic Stem Cell.ES cell)是从早期胚胎中分离得到的多能细胞,在胚胎发育、细胞移植、组织器官构建、药物研制、基因治疗等研究方面发挥着巨大的作用。本文就GFP的特性及其在ES细胞研究中的应用作一简述。 Green fluorescent protein has been used as a convenient and efficient vital marker, which makes its detection convenient without need of exogenous substrates, cell fixation and permealization and has been widely used in cell and molecular biology as a important tool. Embryonic stem cell is multiplutipotent cell which is derived from early embryo and has potential application in the development of embryo, transplantion of organs and tissures, medicine research and gene therapy. The characteristics of GFP and its application in embryonic stem cell research were mainly reviewed in this paper.
作者 杨玉艾 华进联 窦忠英
机构地区 西北农林科技大学国家干细胞工程技术中心陕西分中心
出处 《草食家畜》 2004年第2期23-27,共5页 Grass-Feeding Livestock
基金 国家"863"(2002AA216161) 国家自然科学基金(30200137)
关键词 绿色荧光蛋白 胚胎干细胞 细胞生物学 分子生物学 green fluorescent protein embryonic stem cell
分类号 Q25 [生物学—细胞生物学] S814.8 [农业科学—畜牧学]
  • 相关文献

参考文献22

  • 1Fumthiko N, Masahide Y, Seiji K, et al. Potential Use of Embryonic Stem Cells for the Treatment of Mouse Parkinsonian Models: Improved Behavior by Transplantation of In Vitro Differentiated Dopaminergic Neurons from Embryonic Stem Cells [J]. Stem Cells, 2
  • 2Paparella M, Kolossov E, Fleischmann BK, et al.The use of quantitative image analysis in the assessment of in vitro embryotoxicity endpoints based on a novel embryonic stem cell clone with endoderm-related GFP expression [J]. Toxicol In Vitro, 2002,16(5)
  • 3Hoehn M,Kustermann E,Blunk J,et al. Monitoring of implanted stem cell migration in vivo: a highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat[J]. Proc NatI Acad Sci USA, 2002,99(25):16267-16272.
  • 4Ward, MD, Fluoroscopic technique for doublecontrast knee arthrography[J]. Radiol Technol,May,1979, 50(6): 675-81.
  • 5Tsukahara M, Suemori H, Noguchi S, et al. Novel nucleolar protein, midnolin, is expressed in the mesencephalon during mouse development [J].Gene, 2000,254 : 45-55.
  • 6Gagneten S, Le Y, Miller J, et al. Brief expression of a GFP cre fusion gene in embryonic stem cells allows rapid retrieval of site-specific genomic deletions [J]. Nucleic Acids Res, 1997, 25 (16):3326-31.
  • 7Viswanathan S, Benatar T, Mileikovsky M, et al.Supplementation-dependent differences in the rats of embryonic stem cell self-renewal,differentiation, and apototis [J]. Biotechnol Bioeng, 2003,84(5) :505-517.
  • 8Manuilova ES, Arseneva EL, Khaidarova NV.Influence of regulatory genes of type 1 human immunodeficiency virus on proliferation and differentiation of murine embryonic stem cells[J].Ontogenez, 2003, 34(3): 204-10.
  • 9Asano T,Hanazono Y, Ueda YS, et al. Highly efficient gene transfer into primate embryonic stem cells with a simian lentivirus vector [J]. Mol Ther, 2002, 6(2): 162-168.
  • 10Min JY, Yinke MD, Yang MD, et al. Long-term improvement of cardiac function in rats after infarction by transplantation of embryonic stem cells [J]. J Thorac Cardiovasc Surg, 2003,125:361-369.

同被引文献25

  • 1WANG Dan-bo ZHANG Shu-lan NIU Hui-yan LU Jing-ming.A nude mouse model of endometriosis and its biological behaviors[J].Chinese Medical Journal,2005(18):1564-1567. 被引量：6
  • 2GREEN M, LOEWENSTEIN P M. Autonomous functional domains of chemically synthesized human immunodcficiency virus tat trans-activator protein[J]. Cell, 1988, 55(6): 1179- 1188.
  • 3FRANKEL A D, PABO C O. Cellular uptake of the tat protein from human immunodefieiency virus[J]. Cell, 1988, 55(6) : 1189-1193.
  • 4WENDER P A, MITCHELL D J, PATTABIRAMAN K, et al. The design,synthesis,and evaluation of molecules that enable or enhance cellular uptake:peptoid molecular transporters [J]. Proc Natl Acad Sci U S A, 2000, 97(24) : 13003-13008.
  • 5DOMINGUEZ-SOLA D, YING C Y, GRANDORI C, et al. Non-transcriptional control of DNA replication by c-Myc[J]. Nature, 2007, 448(7152): 445-451.
  • 6HE C, HU H, BRAREN R, et al. c-Myc in the hematopoiet- ic lineage is crucial for its angiogenic function in the mouse embryo[J]. Development, 2008, 135(14): 2487-2477.
  • 7KNOEPFLER P S, ZHANG X Y, CHENG P F, et al. Myc influences global ehromatin strueture[J]. EMBO J, 2006, 25 (12) : 2723-2734.
  • 8VIVES E, BRODIN P, LEBLEU B. A truncated HIV-1 tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus[J]. J Biol Chem, 1997, 272(25): 16010-16017.
  • 9FONSECA S B, PEREIRA M P, KELLEY S O. Recent advances in the use of cell-penetrating peptides for medical and biological applications[J]. Adv Drug Deliv Rev, 2009, 61 (11): 953-964.
  • 10MELIKOV K, CHERNOMORDIK L V. Arginine-rich cell penetrating peptides., from endosomal uptake to nuclear delivery[J]. Cell Mol Life Sci, 2005,62(23) : 2739-2749.

引证文献3

二级引证文献4

草食家畜
2004年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部