根际微生物耦合降解系统的构建及其对蒽污染土壤的修复

CONSTRUCTION OF MICROBIAL COUPLING DEGRADING SYSTEM IN JUTE RHIZOSPHERE AND ITS BIOREMEDIATION OF ANTHRACENE-CONTAMINATED SOIL

将增强型绿色荧光蛋白 (EGFP)标记的黄麻土生根际优势菌Tu 1B分别与蒽高效降解菌An 2和生物表面活性剂产生菌P7-50 进行原生质体电融合 ,得到两株具有荧光标记的融合子Tu An和Tu P .将二融合子接种于植物根际土壤 ,构建根际微生物耦合降解系统 ,在 4 0d时该系统的最大降解率为 96 % .对根际土壤中的Tu An融合子进行了检测 ,结果表明融合子在根际能稳定旺盛生长 .图 3表 4参

Fusant Tu An was obtained through the protoplast electric fusion of Tu 1B, the EGFP labeled dominant bacteria in Jute rhizospheric soil and An 2, the highly effective Anthracene degrading bacteria. At the same time, fusant Tu P was achieved by fusion of Tu 1B and biosurfactant producing bacteria P 7-50 . Two fusants were inoculated into Jute rhizospheric soil, and thus the microbial coupling degrading system was constructed in Jute rhizosphere. The maximum degrading ratio of Anthracene of this system was 96% after 40 d degradation. Work was done to detect the number of fusant Tu An in the Jute rhizospheric soil. The results showed that Tu An could grow steadily in rhizospheric soil. This research provides a new way to resolve the problems of bioaugmentation. Fig 3, Tab 4, Ref 18