摘要
通过制备纳豆提取激酶、纳豆激酶粗酶,对纳豆激酶纤溶性质进行研究,结果表明:纳豆激酶纤溶活性最适pH为8.0,pH6～10溶液中40℃以下基本稳定,pH<5失去纤溶活性;模拟胃环境的酸性条件下,粗酶失去纤溶活性,而纳豆浸提液纤溶活性保持25%;SDS-PAGE电泳显示,胰蛋白酶对纳豆激酶成分没有降解作用;体外溶栓作用表明,纳豆激酶溶解纤维蛋白的方式是直接溶解,而不是通过激活纤溶酶原。
The fibrinolytic Properties of nattokinase was studied by preparing saline extract of natto and crude enzyme of nattokinase, and the results showed nattokinase was stable within pH 6～10 and below 40℃, its optimum pH was 8, but inactivated completely below pH 5. Under simulating acid environment of stomach, the fibrinolytic activity of crude extract of nattokinase lost completely while 25% of the fibrinolytic activity of saline extract of natto retained. The SDS-PAGE results showed nattokinase can not be hydrolyzed by trypsin; nattokinase can hydrolyze fibrin directly rather than by activating plasminogen in vitro.
出处
《江西农业大学学报》
CAS
CSCD
2004年第3期431-434,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
赣财教[2003]88号与赣科发计字[2003]188号资助项目
关键词
纳豆
纳豆激酶
纤溶活性
natto
nattokinase
fibrinolytic activity
