利用多态性片段长度扩增（AFLP）法对印度大吉岭茶树遗传多样性的研究

Genetic Diversity Estimates for Darjeeling Tea Clones Based on Amplified Fragment Length Polymorphism Markers

大吉岭地区的茶树因其特殊的地理环境而具有遗传特点.其不同品系的基因组DNA指纹分析证明有很高的多态性,所以适合以AFLP来研究其无性品系茶树的基因.聚类分析显示,其遗传系统树图谱与先前以形态特征为依据的分类结果一致.大吉岭茶树各品系问的遗传相似性达到70%.在中国类型品种中遗传变异程度较大.在三个种质类型(viz.China,Assam and Cambod type)之间及内部的变异程度分别为63%和36%.

Tea plants growing in Darjeeling area, in addition to all other factors described below, are unique due to their typical geographical isolation. DNA fingerprints revealed a high degree of polymorphism in genomes of different clones, and this demonstrated the suitability of using the Amplified Fragment Length Polymorphism (AFLP) method for genome analysis between closely related plants, such as vegetatively propagated (clonal) populations of tea. Cluster analysis exhibited a dendrogram that closely matched with earlier clonal grouping based on morphological characters. The extent of genetic relatedness between the clones was found to be at 70% level. Results also showed that the genetic variation (Hs) was higher among China type. The variation between and within the three types of clonal populations studied (viz. China, Assam and Cambod type) are 63% and 36% respectively.