新城疫强毒河南株分离鉴定及新城疫二价油乳剂灭活苗研制

Isolation and identification of highly virulent NDV in Henan and preparation of a divalent oil emulsion killed vaccine against ND

从豫北某蛋鸡场病死鸡中分离出一株病毒,经HA试验、HI试验、电镜观察、回归试验确认所分离毒株为新城疫病毒,经对分离株MDT、ICPI的测定表明该分离株为新城疫强毒。对该分离株进行抗原性变异检测和基因型鉴定,证明为基因Ⅶ型新城疫病毒。用新城疫Lasota株和新城疫Ⅶ型毒株制备成新城疫二价油乳剂灭活苗,在河南一些种鸡场和蛋鸡场应用,获得了满意的效果,有效地控制了新城疫的发生和流行。

A Newcastle disease virus was isolated and identified by morphologic examination,hemagglutination test and hemagglutionation-inhibition test.The results showed that the isolated NDV strain was highly virulent pathogenicity.The isolated virus was identified to be a F gene type Ⅶ NDV by antigenic variation examination and gene type identification.ND divalent oil emulusion killed vaccine was made by ND Lasota attenuated strain and ND local isolated strain(F gene type Ⅶ),which produced satisfactory results on some breeding chicken farms and caging hen farms.