摘要
为探索PCR在甘蔗宿根矮化病(RSD)病原菌检测上的应用,对采自广西各蔗区的甘蔗样本进行了PCR检测研究。结果显示:广西各蔗区甘蔗主栽品种及其他大部分引进和自育品种(系)材料均检出RSD病原菌Clavibacterxylisubsp xyli(Cxx),经过热水处理和组培脱毒健康种苗PCR检测呈阴性。PCR检测技术是一项灵敏度高、特异性的分子检测技术。
The aim of this work was to investigate the application of PCR detection in ratoon stunting disease (RSD)of sugarcane.A large number of samples from various cane regions in Guangxi were detected.The results showed that mainly planting varieties from different cane regions were positive for RSD,and most of varieties introduced or bred at home were also positive,but healthy seedcanes through heat treatment or tissue culture were negative.It was concluded that PCR technology was specific,sensitive and rapid.
出处
《西南农业学报》
CSCD
2004年第3期324-327,共4页
Southwest China Journal of Agricultural Sciences
基金
广西科技厅攻关项目(桂科攻0012029)
