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新城疫病毒广西强毒株的分离与鉴定 被引量:5

Isolation and identification of high virulent Newcastle disease viruses in Guangxi
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摘要 从广西某规模化鸡场两个鸡群病死雏鸡中分离到2株有血凝性的病毒,这2株病毒的血凝性均能被新城疫标准阳性血清抑制和中和,经过血凝(HA)试验、血凝抑制(HI)试验、血清中和试验和RT PCR检测结果,确定为新城疫病毒。2株病毒的鸡胚平均死亡时间(MDT)分别为52和54h,脑内致病指数(ICPI)分别为1 975、1 875;鸡胚半数致死量(ELD50)分别为:10-9 38/0 1mL、10-9 68/0 1mL,实验结果证明该2株病毒为新城疫病毒强毒株。 Two isolates of virus were isolated from sick or death chickens of two different flocks in a large chicken farm, which could agglutinate chicken red blood cell. These agglutinations were inhibited and these isolates were neutralized by Newcastle disease virus standard antisera. Those isolates were confirmed to be high virulent Newcastle disease virus based on HA, HI test, antisera neutralization test and the detection results of RT-PCR. 50 % embryo lethal dose (ELD_(50)) of these isolates were 10^(-9.38)/0.1 mL and 10^(-9.68)/0.1mL,separately,and mean death time (MDT) of chicken embryos of these isolates were 52, 54 h,separately,Intracerebral pathogenicity index (ICPI) of these isolates in one day old SPF chicken were 1.975, 1.875,separately. The results demonstrated that 2 isolates of virus were high virulent Newcastle disease virus.
机构地区 广西兽医研究所
出处 《西南农业学报》 CSCD 2004年第3期390-392,共3页 Southwest China Journal of Agricultural Sciences
基金 广西科技攻关项目资助部分内容(桂科攻0235001-4)
关键词 新城疫病毒 强毒株 分离 鉴定 Newcastle disease virus virulent strain isolation and identification
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  • 1孙一敏,于秀俊,柏佳宁,孙继国,赵宝华.NDV HeB02分离株的生物学特性及其F基因的克隆与序列测定[J].微生物学通报,2004,31(4):5-8. 被引量:6
  • 2唐小飞,谢芝勋,刘加波,庞耀珊,邓显文.新城疫病毒广西分离株F基因的克隆和序列分析[J].中国兽医科技,2005,35(5):333-340. 被引量:10
  • 3刘加波,谢芝勋,唐小飞,庞耀珊,邓显文.10株新城疫病毒广西分离株HN蛋白基因的克隆与序列分析[J].畜牧与兽医,2005,37(7):6-10. 被引量:9
  • 4颜健华,刘桂生,卜绍全,刘以恒,黄玉梅,施运潭,刘玉良.新城疫病毒强弱毒株PCR-RFLP鉴别方法的建立[J].动物医学进展,2007,28(4):1-5. 被引量:5
  • 5国际兽疫局.诊断试验和疫苗标准手册[M].第3版.农业部畜牧兽医局编译.北京:中国农业科学技术出版社,1996.
  • 6[2]Wemers CD,de Henau S,Neyt C,et al.The hemagglutininneuraminidase (HN) gene of Newcastle disease virus strain Italien(ndv Italien):comparison with HNs of other strains and expression by a vaccinia recombinant.Arch Virol,1987;97(1-2):101~113
  • 7[3]Kaleta,E F,and Baldauf C.Newcastle disease in free-living and pet birds.In Alexader D J(ed.)Newcastle disease.Kluwer Academic Publishers,Bosto,M A,1988:197~246
  • 8[8]Xie Z,Fadl A A,Girshick T,et al.Amplification of Avian Reovirus RNA Using the Reverse Transcriptase-Polymerase Chain Reaction[J].Avian Diseases,1997;41:654~660
  • 9卡尔尼克BW.禽病学[M]:第10版[M].北京:农业出版社,1999.806-815.
  • 10Samal S K, Collins P L. RNA replication by a minigenome of respiratory syncytial virus (RSV) does not obey "the rule of six "describe for other paramyxoviruses [J]. J Virol, 1996, 70: 5075-5082.

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