摘要
从广西某规模化鸡场两个鸡群病死雏鸡中分离到2株有血凝性的病毒,这2株病毒的血凝性均能被新城疫标准阳性血清抑制和中和,经过血凝(HA)试验、血凝抑制(HI)试验、血清中和试验和RT PCR检测结果,确定为新城疫病毒。2株病毒的鸡胚平均死亡时间(MDT)分别为52和54h,脑内致病指数(ICPI)分别为1 975、1 875;鸡胚半数致死量(ELD50)分别为:10-9 38/0 1mL、10-9 68/0 1mL,实验结果证明该2株病毒为新城疫病毒强毒株。
Two isolates of virus were isolated from sick or death chickens of two different flocks in a large chicken farm, which could agglutinate chicken red blood cell. These agglutinations were inhibited and these isolates were neutralized by Newcastle disease virus standard antisera. Those isolates were confirmed to be high virulent Newcastle disease virus based on HA, HI test, antisera neutralization test and the detection results of RT-PCR. 50 % embryo lethal dose (ELD_(50)) of these isolates were 10^(-9.38)/0.1 mL and 10^(-9.68)/0.1mL,separately,and mean death time (MDT) of chicken embryos of these isolates were 52, 54 h,separately,Intracerebral pathogenicity index (ICPI) of these isolates in one day old SPF chicken were 1.975, 1.875,separately. The results demonstrated that 2 isolates of virus were high virulent Newcastle disease virus.
出处
《西南农业学报》
CSCD
2004年第3期390-392,共3页
Southwest China Journal of Agricultural Sciences
基金
广西科技攻关项目资助部分内容(桂科攻0235001-4)
关键词
新城疫病毒
强毒株
分离
鉴定
Newcastle disease virus
virulent strain
isolation and identification