山核桃基因组DNA提取方法的比较研究

Study on Isolation Methods of Genomic DNA in Carya Cathayensis

植物体内所含的蛋白质、单宁、酚类、多糖及色素等次生代谢物质影响TaqDNA聚合酶的活性,是导致PCR扩增反应失败的主要因素。以山核桃嫩叶为材料,对分别用经过改良的SDS法、改良的CTAB法、简易CTAB法及改良的高盐低pH法提取的基因组DNA进行检测比较,结果显示,改良的SDS法更适合于山核桃基因组DNA的提取,此方法提取的DNA能很好地用于PCR扩增。

High content of protein,phenol,tannin,pigment and polysaccharide in plant tissues usually affect the activity of Taq DNA polymerase and Lead to failure of PCR reaction.The methods of improved CTAB,rapid CTAB,improved SDS and improved low pH medium were used for genomic DNA isolation in Carya cathayensis.The results indicated that the method of improved SDS was the most suitable one for Carya cathayensis genomic DNA isolation and the DNA isolated can be used for PCR reaction.