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水稻插入突变库构建研究进展 被引量:14

Progress in Rice Insertional Mutagenesis
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摘要 水稻是单子叶植物基因组研究的一种模式植物 ,其全基因组测序已经完成 ,在此基础上开展功能基因组的研究。水稻插入突变体库的建立是功能基因组研究的一个重要内容 ,在此基础上也能进行正向遗传学及反向遗传学的研究。水稻插入突变体库构建的方法有T DNA插入突变、Ac Ds系统插入突变、Tos1 7插入突变。 Rice (Oryza sativa) is a model plant in genomics research.Its functional genome research is becoming a topic and attractive theme in plant genome program,based on its completed genomic sequencing.Insertional mutagenesis has the advantage that the inserted element acts as a tag for gene identification,and it is convenience for both forward and reverse genetics research.Insertional mutagenesis using T\|DNA,Ac/Ds and Tos17 are commonly methods to creating a mutant library in rice.Here we summarize these strategies as well as the progress in the functional genomics of rice.
出处 《中国生物工程杂志》 CAS CSCD 2004年第6期48-53,共6页 China Biotechnology
基金 国家转基因植物专项基金资助项目 (ZJY A 0 8) 国家"863"计划资助项目( 2 0 0 1AA2 2 5 0 12 )
关键词 水稻 插入突变 突变体库 功能基因组 Rice Insertional mutagenesis Gene tags
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参考文献44

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二级参考文献30

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