免疫微阵列同步多元分析系统的建立及其初步应用

Development and primary application of a simultaneous multiplexing immunoassay system

本研究建立了一种基于抗体微阵列芯片的用于多个生物样品同步检测的分析方法。通过制备条件实验,确定了抗体在微阵列芯片基片表面的多项固化条件;通过鉴定及应用条件实验,确定了抗体微阵列分析系统用于三种肿瘤蛋白质检测的方法学技术指标。本分析系统体现了微阵列检测的高通量性、夹心免疫分析的高特异性和亲合素-生物素系统的高灵敏度。实验及结果分析表明,该系统具有较强的应用价值及开发潜力。

A simultaneous multiplexing immunoassay system has been developed which allow to detect multiple analytes based on antibody microarray. Technical data are optimized for the manufacture of the arrays. This system has been applied to determine three tumor markers: α-fetoprotein (AFP ), carcinoma embryonic antigen (CEA), and human chorionic gonadotropin beta (β-HCG). Our method has the advantage of showing the high throughput of microarray, specificity of 'sandwich' immunoassay and sensitivity of avidin-biotin system. Simultaneous multiplexing immunoassay based on our antibody microarrays may find feasible application in the measurement of series markers in oncology and other areas of bioscience.