C/N对细菌产氢发酵类型及产氢能力的影响

EFFECT OF C/N ON BACTERIUM PRO-HYDROGEN FERMENT TYPE AND PRO-HYDROGEN ABILITY

反应基质中的C/N比作为影响因子,参与细菌的产能代谢过程,主要作用于微生物的自身合成代谢过程和有机物在微生物体内的生物氧化过程。乙醇型发酵过程中由于物质和能量转化间高度平衡细胞合成代谢处于较低的水平,而丁酸型发酵过程中,由于NADH+H+参与细胞合成代谢。所以发酵基质内C/N比过低,过剩的N源物质进一步促进了微生物细胞的合成代谢。并且导致的发酵类型向丁酸型发酵转变的现象,是微生物种群维持"内平衡"的适应性结果。分析认为反应基质中的C/N比作为影响因子,是作用于系统发酵产氢过程稳定性的主要因素之一。在试验及生产过程中,为了得到最佳产氢发酵类型—乙醇型发醇,应严格控制反应系统底物环境内C/N≥200,将微生物细胞合成代谢过程控制在较低的水平,在提高系统产氢能力及其稳定性的同时,降低系统剩余污泥的产生量。

Effect factor-C/N of the reaction basic matter is associated with pro-energy metabolism process of bacterium. C/N mainly reacts on synthetic metabolism and bio-oxidizing of bacterium themselves. The high level balance between matter and energy transferring makes cell synthetic metabolic a lower level in Ethanol type fermentation (ETF), and NADH+H^+ participates in cell synthetic metabolism in Butyric and type fermentation (BATF), so the excess N fountainhead matter will promote bacterium synthetic metabolism when C/N is low in foundation matter. The results showed that C/N in reaction foundation was one of the main factors to affecting the stability of the process of system ferment pro-hydrogen and the lower C/N in the system led ferment type to BATF. The bacterium community kept internal equilibrium mechanism. C/N ≥200 in the bottom environment of reaction system should be strictly controlled, and the synthetic metabolic with a lower level in bacterium cells was maintained, so as to improve pro-hydrogen ability and stability in reaction system , and decrease surplus sludge products.