摘要
利用苜蓿无菌苗的子叶、下胚轴作为外植体,在附加2.0 mg·L^(-1)KT 和0.5 mg·L^(-1)2,4-D 的 LS 培养基上诱导愈伤组织的发生,经悬浮培养产生胚状体,在含有活性炭的分化培养基上诱导根芽分化并再生植株。以根芽分化及胚状体形成的不同时期培养物为材料,分别提取可溶性蛋白,进行 SDS-聚丙烯酰胺凝胶电泳及电泳扫描,初步得到分子量为110 kD 和88 kD 的蛋白质,它们可能分别与不定芽和不定根分化有关。
Cotyledon and hypocotyl segments of aseptic seedlings of alfalfa were cultured on LS media with 2.0 mg·L^(-1)KT and 0.5 mg·L^(-1)2,4-D,callus were induced and embryoids were formed by sus- pension culture.Adventitious roots and buds were induced and regenerated plants were obtained on differ- entiation media.Total soluble proteins were extracted from the cultured meterials in different stages of organogenesis and somatic embryogenesis.It was preliminarily determined by SDS-PAGE and scanning analysis that the protein of molecular weight of 110 kD and that of molecular weight of 88 kD were the specific proteins related to adventitious bud and root differentiation,respectively.
基金
农业部植物生理生化重点开放实验室
关键词
苜蓿
组织培养
不定根
蛋白
tissue culture
alfalfa
adventitious root
adventitious bud
protein
