消化间期移行性复合运动对胆道调节作用的实验研究

Experimental study on regulation of bile duct motility by gastrointestinal migrating motor complex

目的 探讨消化间期移行性复合运动 (MMC)与胆道运动的相互关系及两者间的相互调节作用 ,进一步阐明MMC的发生机制 ,并为胆石症的防治提供理论依据。方法 将 4 5只成年豚鼠分为对照组 ,普卡比利组和地巴唑组 ,在胃窦、十二指肠上段、十二指肠中段、胆囊 (GB)及Oddi括约肌(SO)等五处肌肉层内埋置银丝电极 ,用八导生理记录仪记录该部位肌电活动 ,胃内分别给予普卡比利和地巴唑后 ,观察肌电变化及MMC与GB、SO运动的关系。结果 各组动物给药前五个记录点的肌电活动频率和强度呈正相关 (P <0 .0 5 )。胃肠MMC的肌电活动被普卡比利增强后 ,GB、SO部位的肌电活动相应地加强。胃肠MMC的肌电活动被地巴唑抑制后 ,GB、SO部位的肌电活动相应地被抑制 (P <0 .0 5 )。结论 胃肠肌电活动的改变能引起胆道肌电活动的相应变化 ,提示胃肠MMC对胆道运动有调节作用。

Objective In order to elucidate the mechanism of migrating motor complex (MMC) in prophylaxis and treatment of cholelithiasis,the relationship and interaction between MMC and the bile duct motility were investigated. Methods Forty five adult guinea pigs were allocated into three groups with 15 each. The guinea pigs in control,prucalopride and dibazol groups were fed with saline (0.15 ml/100 g),prucalopride (0.01 mg/100 g) or dibazol (0.6 mg/100 g),respectively. The myoelectrical activities of antrum,duodenum,gallbladder(GB) and sphincter of Oddi(SO) of guinea pigs were recorded simultaneously before and after administration via implanted silver electrodes of the muscle layer. Results Positive correlation in frequency and strength of spontaneous myoelectrical activity was demonstrated among antrum,duodenum,GB and SO before administration( P <0.05). The myoelectrical activity of GB and SO was enhanced correspondingly after the MMC was provocated by prucalopride,while it was diminished after the MMC was restrained by dibazol ( P <0.05). Conclusion The changes of myoelectrical activity of antrum and duodenum can modulate the myoelectrical activity of GB and SO. The present study indicates that MMC had potential effect on bile duct motility.