RP-HPLC测定不同产地青木香和细辛中马兜铃酸A的含量

Determination of aristolochic acid A in Radix Aristolociae and Herba Asari by RP-HPLC

目的 :测定不同地区市售青木香、细辛中马兜铃酸A的含量 ,用于指导临床用药和相关研究。方法 :应用高效液相色谱法 ,选用AlltechC18色谱柱 (4.6mm× 2 5 0mm ,5 μm) ,甲醇 水 醋酸 (6 8∶32∶1)为流动相 ,流速为 1.0mL·min-1,柱温为 35℃ ,紫外检测波长为 390nm。结果 :马兜铃酸A在 0 .119～ 1.89μg有良好的线性关系 ,平均加样回收率为 10 1.8% ,RSD为 2 .0 9%。不同产地青木香中马兜铃酸A的含量在 0 .916 9～ 1.90 76mg·g-1,其中以河北安国产青木香的含量较高 (1.90 76mg·g-1) ;细辛中马兜铃酸A的含量在 0～ 35 1g·g-1,其中以吉林市售品含量较高。结论 :本方法精密度高 ,重现性好 ,可用来含马兜铃酸类中药材中马兜铃酸A的含量测定 ;含马兜铃酸的中药中以关木通中马兜铃酸A的含量最高 ,青木香中的含量约为关木通中的含量的 1/3,细辛的含量最低。

Objective: To develop a HPLC method to determine the contents of aristolochic A in aristolochia debilis and Asarum spp..Method: methanol-water-formic acid extracts were sperated on an alltech C_ 18 column with methanol-water-acetic acid (68∶32∶1) as mobile phase. The flow rate was 1.0 mL·min -1 . UV detection wavelength was 390 nm. Column temperature was 35 ℃. Result: Aristolochic acid A was separated well. The relationship of injection amounts and peak areas was linear ( r =0.999 9) the range of 0.12～1.89 μg·g -1 and the recovery rate was 101.8% ( n =5). 11 samples of aritolochia debilis which bought from different areas in China were deterined, and the contents of aristolochic acid A varied from 0.9 to 2 mg·g -1 . The difference of the contents in Asarum spp. was obvious. The highest is 0.35, and arisolochic acid A couldn't be detected in on sample.