摘要
目的 :研究葛根素与大豆苷元对血管平滑肌细胞增殖的抑制作用 ,并探讨其机制。方法 :分离培养人脐动脉平滑肌细胞 ,不同浓度葛根素、大豆苷元与细胞共孵育 ,使用MTT法检测细胞活性 ,同时用Rt PCR法检测Survivin ,Bcl xl,Bax ,GapdhmRNA的表达。结果 :葛根素与大豆苷元都可降低细胞增殖活性 ,升高Bax/Gapdh/Bcl xl/Gapdh的比值。与葛根素组相比 ,相同浓度的大豆苷元组细胞活性较低 ,Bax/Gapdh/Bcl xl/Gapdh比值较高。结论 :与葛根素相比 ,大豆苷元抑制血管平滑肌细胞增殖的作用更强。
Objective: To observe the different effects of Puerarin and Daidzein on the expression of proliferating vascular smooth muscle cells, and to discuss the mechanism. Method: MTT was used to detect the state of VSMC(vascular smooth muscle cell) activity. The expression levels of Survivin,Bcl-xl, Bax and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNA (mRNA) were analyzed quantitatively by reverse transcriptase polymerase chain reaction (Rt-PCR). Result: Compared with Puerarin groups, VSMC activity in daidzein groups was lower, and the ratio of Bax/Gapdh/ Bcl-xl/Gapdh was higher. Conclusion:The inhibition effect of daidzein on VSMC proliferation is stronger than that of puerarin.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2004年第5期437-440,共4页
China Journal of Chinese Materia Medica
基金
北京市科委重点实验室项目 (95 3 85 0 70 0 4)