3种中药体外抗巨细胞病毒效应的比较

Experimental study on antivirus activity of traditional Chinese medicine

目的 :探讨中药金叶败毒注射液、板蓝根注射液、路边青注射液抗人巨细胞病毒 (HCMVAD16 9)作用的差异。方法 :采用细胞病变 (CPE)抑制法观察这 3种中药对HCMVAD16 9毒株的抑制作用。使用MTT法检测细胞的损伤程度 ,并利用A值评价 3种中药的抗HCMV的效应。结果 :金叶败毒注射液的半数中毒浓度 (TD50 )为 2 0g·L-1,半数有效浓度 (IC50 )为 5 .6 5g·L-1,治疗指数 (TI)为 3.5 4 ;板蓝根注射液的TD50 为 10 .2 3g·L-1,IC50 为 3g·L-1,TI为 3.4 1;路边青注射液TD50 为 2 0 .2 3g·L-1,IC50 为 5 .71g·L-1,TI为 3.5 4。 3种中药均具有抗HCMV的作用 ,且随药物浓度的增高其作用也增强。但这 3种中药抗HCMV作用的差异无显著性 (P >0 .0 5 )。结论 :在 1∶2 0 0 ,金叶败毒注射液、板蓝根注射液、路边青注射液均具有较强的抗病毒作用 ,都是理想的抗HCMV中药。

Objective: To investigate the difference of anticytomegaloviral activity of three kinds of traditional Chinese medicines which are the injections of Jinye Baidu, Radix Isatidis and Indigowoa in vitro. Method:The inhibitory activity of three traditional Chinese medicines against human cytomeglovirus( HCMV AD169) infected human embryo lung fibroblasts (HELF) was observed by cytopathic effect method (CPE) and MTT method in vitro. According their value of A , anticytomegaloviral activity has evaluated. Result:Experimental study in vitro showed that the 50% toxicity dose (TD_ 50 ) of Jinye Detoxifying, Radix Isatidis root and Indigowoa were 20, 10.23, 20.23 g·L -1 respectively;the 50% inhibitory concertration (IC_ 50 ) were 5.65, 3, 5.71 g·L -1 respectively;the therapeutic index (TI) were 3.54,3.41 and 3.54 respectively. It suggested that three traditional Chinese medicines had anticytomeglovirus activity and their effect increased with their concentration. Conclusion:Three traditional Chinese medicines of the parenteral solution of Jinye Detoxifying, Radix Isatidis root and Indigowoa have antiviral activity when they are diluted in 1∶200. They are safe and valuable drug for inhibiting cytomeglovirus infection.