RP-HPLC测定母体及胎儿血浆中罗哌卡因和布比卡因的药物浓度

Determination of concentration of ropivacaine and bupivacaine in maternal and fetus plasma by reverse phase high performance liquid chromatography

目的 :建立罗哌卡因、布比卡因血浆药物浓度的反相高效液相 (RP HPLC)测定方法 ,并测定其母体和 (或 )胎儿血药浓度 ,为临床合理用药提供参考。方法 :采用AgilentHPLC系统 ;色谱柱 :Dikma C18(5 μm ,4 .6× 15 0mm)。以罗格列酮作为内标 ,流动相为磷酸二氢钠缓冲液 (10mmol·L- 1,pH3.0 )∶乙腈 =78∶2 2 ,流速 1.0mL·min- 1,λ =2 10nm。结果 :罗哌卡因线性关系为Y =0 .0 2 95X -0 .0 2 98(n =7,r =0 .9998) ;最低检测浓度为 0 .0 1mg·L- 1;平均回收率为 99.82 % ;日内、日间RSD分别小于 2 .4 7% ,3.75 % ;布比卡因线性关系为Y =0 .0 2 87X + 0 .0 2 71(n =7,r =0 .9998) ;最低检测浓度为 0 .0 1mg·L- 1;平均回收率为 10 1.0 1% ;日内、日间RSD分别小于 2 .6 9% ,4 .75 %。测定 6 0个血样 ,罗哌卡因浓度为 0 .15～ 0 .7mg·L- 1,布比卡因为 0 .1～ 0 .5 8mg·L- 1。结论 :RP HPLC法简便、灵敏、准确 ,可以用来测定临床血样中罗哌卡因、布比卡因的药物浓度 ,可为临床合理用药提供参考。

AIM: To establish reverse phase high performance liquid chromatography (RP-HPLC) method to determine the concentration of ropivacaine and bupivacaine in maternal and fetus plasma. METHODS: Agilent HPLC instrument was used with the column: Dikma-C 18(5 μm,4.6×150 mm). Internal standard was rosiglitazone. The mobile phase was composed of NaH 2PO 4(10 mmol·L -1,pH 3.0)∶CH 3CN(78∶22,V/V).Flow rate was 1.0 mL·min -1.Detection wavelength was 210 nm. RESULTS: The linear coefficient relation of ropivacacine and bupivacaine were Y=0.0295X- 0.0298 and Y=0.0287X+0.0271(r=0.9998,n=7). The minimum detection limit of ropivacaine and bupivacaine were both 0.01 mg·L -1. The linear ranges were both (0.01-5.0) mg·L -1. The average recovery rates of method were 99.82% and 101.01%. The coefficient variations of intra- and inter-day of ropivacaine for 0.1, 1, 5 mg·L -1 were less than 2.47% and 3.75%. The coefficient variations of intra- and inter-day of bupivacaine for 0.1, 1, 5 mg·L -1 were less than 2.69% and 4.75%. The concentration of sixty samples of ropivacaine was 0.15-0.7 mg·L -1, and that of bupivacaine was 0.1-0.58 mg·L -1. CONCLUSION: The RP-HPLC method is simple, sensitive and accurate. It is applicable to determine the concentration of ropivacaine and bupivacaine.