摘要
目的 :以原位杂交法检测PPL13在人胎盘组织的表达情况。方法 :用DIG标记的PPL13有义RNA探针及反义RNA探针与胎盘组织切片进行原位杂交。结果 :反义RNA探针杂交标本显色 3h ,显微镜下可见胎盘毛细血管内皮细胞中出现棕色沉淀物 ,滋养层细胞中未见棕色沉淀物 ;而有义RNA探针杂交标本显色 2 4h仍无棕色沉淀物出现。结论 :PPL13在人胎盘内皮细胞中特异性表达 ,提示PPL13可能与胎盘的屏障作用有关。
Objective: Identify the expression of PPL13 in the cells of placenta. Methods: ISH (in situ hybridization) was used to examine the expression of PPL13 in the human placenta. Results: The transcripts of PPL13 predominantly expressed in the endothelial cells of the human placenta. Conclusion: The result suggests that PPL13 possibly play a role in the function of placental barrier.
出处
《湖北省卫生职工医学院学报》
2004年第1期11-13,共3页
Journal of Hubei Medical Staff College